Recombinant
RabMAb

Recombinant Anti-PER2 antibody [EPR11381(2)] (Alexa Fluor® 594) (ab217086)

Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)] (Alexa Fluor® 594)
    See all PER2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2 (Alexa Fluor® 594)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PER2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15055

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab217086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

Target

  • Function

    Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.
  • Tissue specificity

    Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.
  • Involvement in disease

    Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
  • Sequence similarities

    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications

    Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt
  • Database links

  • Alternative names

    • Circadian clock protein PERIOD 2 antibody
    • FASPS antibody
    • FASPS1 antibody
    • hPER 2 antibody
    • hPER2 antibody
    • KIAA0347 antibody
    • OTTHUMP00000164476 antibody
    • PER 2 antibody
    • PER2 antibody
    • PER2_HUMAN antibody
    • Period 2 antibody
    • Period 2 isoform 1 antibody
    • Period circadian clock 2 antibody
    • Period circadian protein 2 antibody
    • Period circadian protein homolog 2 antibody
    • Period homolog 2 (Drosophila) antibody
    • Period homolog 2 antibody
    • Period, Drosophila, homolog of, 2 antibody
    • Period2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab217086 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab217086, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 594 (ab208568) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 610/20 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab217086 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab217086.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up