Anti-PER2 antibody - N-terminal (ab227727)
Key features and details
- Rabbit polyclonal to PER2 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PER2 antibody - N-terminal
See all PER2 primary antibodies -
Description
Rabbit polyclonal to PER2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human PER2 (N terminal). The exact sequence is proprietary.
Database link: O15055 -
Positive control
- WB: PER2-transfected HEK-293T whole cell lysate. IHC-P: Adult mouse retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 137 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 137 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
Tissue specificity
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung. -
Involvement in disease
Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
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Database links
- Entrez Gene: 8864 Human
- Entrez Gene: 18627 Mouse
- Omim: 603426 Human
- SwissProt: O15055 Human
- SwissProt: O54943 Mouse
- Unigene: 58756 Human
- Unigene: 218141 Mouse
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Alternative names
- Circadian clock protein PERIOD 2 antibody
- FASPS antibody
- FASPS1 antibody
see all
Images
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Paraffin-embedded adult mouse retina tissue stained for PER2 (green) using ab227727 at 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3, stained by a beta Tubulin 3 antibody at 1/250 dilution.
Blue:DAPI -
All lanes : Anti-PER2 antibody - N-terminal (ab227727) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PER2-transfected HEK-293T whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 137 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab227727 has been referenced in 2 publications.
- Chen X et al. Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production. Front Cell Dev Biol 9:636802 (2021). PubMed: 33869182
- Németh V et al. Expression Patterns of Clock Gene mRNAs and Clock Proteins in Human Psoriatic Skin Samples. Int J Mol Sci 23:N/A (2021). PubMed: 35008548