Product nameAnti-PER2 antibody - N-terminal
See all PER2 primary antibodies
DescriptionRabbit polyclonal to PER2 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human PER2 (N terminal). The exact sequence is proprietary.
Database link: O15055
- WB: PER2-transfected HEK-293T whole cell lysate. IHC-P: Adult mouse retina tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 137 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionComponent of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
Tissue specificityWidely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.
Involvement in diseaseDefects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
Sequence similaritiesContains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
modificationsPhosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.
Cellular localizationNucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
- Information by UniProt
- Circadian clock protein PERIOD 2 antibody
- FASPS antibody
- FASPS1 antibody
Paraffin-embedded adult mouse retina tissue stained for PER2 (green) using ab227727 at 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3, stained by a beta Tubulin 3 antibody at 1/250 dilution.
All lanes : Anti-PER2 antibody - N-terminal (ab227727) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PER2-transfected HEK-293T whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 137 kDa
ab227727 has not yet been referenced specifically in any publications.