Key features and details
- Mouse monoclonal [4B9D7] to PER3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-PER3 antibody [4B9D7]
See all PER3 primary antibodies
DescriptionMouse monoclonal [4B9D7] to PER3
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PER3 aa 723-954. (Purified from E.coli).
DSTSKQTRSAGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQPCCP SAASSPHTSSPTFPPAAMVPSQAPYLVPAFPLPAATSPGREYAAPGTAPE GLHGLPLSEGLQPYPAFPFPYLDTFMTVFLPDPPVCPLLSPSFLPCPFLG ATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEAQSEGHPFITSRSS SPLQLNLLQEEMPRPSESPDQMRRNTCPQTEY
Database link: P56645
- Human PER3 (aa723-954) recombinant protein; PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and rectum cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab201940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 132 kDa.|
|ICC/IF||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionComponent of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme.
Sequence similaritiesContains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
modificationsPhosphorylation appears to require association with PER1 and translocation to the nucleus.
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS.
- Information by UniProt
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Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution + Human PER3 (aa723-954) recombinant protein
Predicted band size: 132 kDa
Expected MWt is 50.7 kDa.
All lanes : Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 132 kDa
(Expected MW is 50.7 kDa)
Immunofluorescent analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution (green) compared to a negative control (red).
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.
ab201940 has been referenced in 1 publication.
- Knarr M et al. miR-181a modulates circadian rhythm in immortalized bone marrow and adipose derived stromal cells and promotes differentiation through the regulation of PER3. Sci Rep 9:307 (2019). PubMed: 30670712