Key features and details
- Rabbit polyclonal to PER3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PER3 antibody
See all PER3 primary antibodies
DescriptionRabbit polyclonal to PER3
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: HeLa whole cell lysate (ab150035); mouse skeletal muscle tissue lysate. IHC: Human ovarian cancer and colon cancer tissue lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).|
FunctionComponent of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme.
Sequence similaritiesContains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
modificationsPhosphorylation appears to require association with PER1 and translocation to the nucleus.
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS.
- Information by UniProt
- 2810049O06Rik antibody
- Cell growth inhibiting gene 13 protein antibody
- Cell growth-inhibiting gene 13 protein antibody
All lanes : Anti-PER3 antibody (ab224594) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse skeletal tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 132 kDa
Observed band size: 132 kDa
Paraffin-embedded human ovarian cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PER3 (green) using ab224594 at 1/100 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224594 has not yet been referenced specifically in any publications.