Overview

  • Product name
    Anti-PER3 antibody [EPR13038]
    See all PER3 primary antibodies
  • Description
    Rabbit monoclonal [EPR13038] to PER3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human PER3 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P56645

  • Positive control
    • Fetal heart and HeLa and K562 lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme.
    • Sequence similarities
      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Post-translational
      modifications
      Phosphorylation appears to require association with PER1 and translocation to the nucleus.
      Ubiquitinated.
    • Cellular localization
      Cytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810049O06Rik antibody
      • Cell growth inhibiting gene 13 protein antibody
      • Cell growth-inhibiting gene 13 protein antibody
      • Circadian clock protein PERIOD 3 antibody
      • GIG13 antibody
      • gPER3 antibody
      • Growth inhibiting protein 13 antibody
      • HGNC:8847 antibody
      • hPER3 antibody
      • mPer3 antibody
      • Per3 antibody
      • PER3_HUMAN antibody
      • Period 3 antibody
      • Period circadian clock 3 antibody
      • Period circadian protein 3 antibody
      • Period circadian protein homolog 3 antibody
      • Period homolog 3 (Drosophila) antibody
      • Period homolog 3 antibody
      • Period homolog 3, Drosophila antibody
      • Period3 antibody
      see all

    Images

    • All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (purified)

      Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : Human fetal heart lysates
      Lane 3 : Rat heart lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 132 kDa
      Observed band size: 132 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (purified)

      Lane 1 : Mouse kidney lysates
      Lane 2 : Mouse heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 132 kDa
      Observed band size: 132 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 132 kDa

    References

    This product has been referenced in:
    • Meier D  et al. Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes. PLoS One 10:e0137229 (2015). WB . Read more (PubMed: 26361389) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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