Recombinant Anti-PER3 antibody [EPR13038] (ab177482)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13038] to PER3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PER3 antibody [EPR13038]
See all PER3 primary antibodies -
Description
Rabbit monoclonal [EPR13038] to PER3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal heart and HeLa and K562 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13038 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
Target
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Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylation appears to require association with PER1 and translocation to the nucleus.
Ubiquitinated. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS. - Information by UniProt
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Database links
- Entrez Gene: 8863 Human
- Entrez Gene: 18628 Mouse
- Entrez Gene: 78962 Rat
- Omim: 603427 Human
- SwissProt: P56645 Human
- SwissProt: O70361 Mouse
- SwissProt: Q8CJE2 Rat
- Unigene: 162200 Human
see all -
Alternative names
- 2810049O06Rik antibody
- Cell growth inhibiting gene 13 protein antibody
- Cell growth-inhibiting gene 13 protein antibody
see all
Images
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All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (purified)
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 132 kDa
Observed band size: 132 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (purified)
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 132 kDa
Observed band size: 132 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-PER3 antibody [EPR13038] (ab177482) at 1/1000 dilution (unpurified)
Lane 1 : Fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : K562 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 132 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab177482 has been referenced in 3 publications.
- Li Q et al. Circadian Rhythm Gene PER3 Negatively Regulates Stemness of Prostate Cancer Stem Cells via WNT/ß-Catenin Signaling in Tumor Microenvironment. Front Cell Dev Biol 9:656981 (2021). PubMed: 33816508
- Crosby P et al. Insulin/IGF-1 Drives PERIOD Synthesis to Entrain Circadian Rhythms with Feeding Time. Cell 177:896-909.e20 (2019). PubMed: 31030999
- Meier D et al. Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes. PLoS One 10:e0137229 (2015). WB . PubMed: 26361389