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  1. Link

    percp-cd11b-antibody-dcis118-ab91145.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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PerCP Anti-CD11b antibody [DCIS1/18] (ab91145)

  • Datasheet
  • SDS
Reviews (7)Q&A (1)References (6)

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Abpromise

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Key features and details

  • PerCP Mouse monoclonal [DCIS1/18] to CD11b
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG2a

You may also be interested in

Protein
Recombinant Human CD11b protein (ab126010)

View more associated products

Overview

  • Product name

    PerCP Anti-CD11b antibody [DCIS1/18]
    See all CD11b primary antibodies
  • Description

    PerCP Mouse monoclonal [DCIS1/18] to CD11b
  • Host species

    Mouse
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm
  • Specificity

    Integrin heterodimer of MW 165,95kD which comprises the receptor (CR3 am chain, CD11b/ CD18) for the complement component C3i. The CR3 receptor is expressed as a surface molecule by circulating monocytes and granulocytes and also by dendritic reticulum cells in sections of lympoid tissue. Expression is increased on activated granulocytes.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD11b. Immunogen: Dendritic cells derived from human monocytes.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    DCIS1/18
  • Isotype

    IgG2a
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Isotype control

    • PerCP Mouse IgG2a [MOPC-173] - Isotype Control (ab209397)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • Unigene: 172631 Human
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab91145? Please let us know so that we can cite the reference in this datasheet.

    ab91145 has been referenced in 6 publications.

    • Salimi M  et al. Activated innate lymphoid cell populations accumulate in human tumour tissues. BMC Cancer 18:341 (2018). Flow Cyt ; Human . PubMed: 29587679
    • Pandya H  et al. Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. Nat Neurosci 20:753-759 (2017). PubMed: 28253233
    • Salimi M  et al. Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines. J Allergy Clin Immunol 140:1090-1100.e11 (2017). PubMed: 28115217
    • Hams E  et al. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. Proc Natl Acad Sci U S A 111:367-72 (2014). PubMed: 24344271
    • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . PubMed: 24388011
    • Griffin DO & Rothstein TL A small CD11b+ human B1 cell subpopulation stimulates T cells and is expanded in lupus. J Exp Med 208:2591-8 (2011). WB ; Human . PubMed: 22110167

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rabbit Cell (blood leucocytes)
    Specification
    blood leucocytes
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2012

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Pig Cell (blood leucocytes)
    Specification
    blood leucocytes
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2012

    Question

    Is product ab91145 cross-reactive with mouse CD11b?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 04 2012

    Answer

    Unfortunately, the exact epitope recognized by product ab91145 has not yet been mapped, so it is not possible to accurately predict cross-reactivity of this Ab with mouse CD11b. However, the human and mouse CD11b sequences show approximately ˜70% homology.

    Unfortunately, researchers have previously reported poor results using this Ab with mouse, rat, monkey, and dog samples - please see the Abreviews below for this data.

    While it is possible that product ab91145 may cross-react with mouse CD11b, this is not guaranteed, and significant optimization may be necessary. The information and results we currently have suggest that this product does not provide good results with mouse samples.

    Read More

    Abcam Scientific Support

    Answered on Jun 04 2012

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive macrophages
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Dog Cell (blood leucocytes)
    Specification
    blood leucocytes
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 25 2011

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Blood cells)
    Specification
    Blood cells
    Preparation
    Cell harvesting/tissue preparation method: Lysis of red blood cells
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    Neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2010

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Monkey Cell (Blood cells)
    Specification
    Blood cells
    Preparation
    Cell harvesting/tissue preparation method: Lysis of red blood cells
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    Monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2010

    Flow Cytometry abreview for Anti-CD11b antibody [DCIS1/18] (PerCP)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (leukocytes)
    Specification
    leukocytes
    Preparation
    Cell harvesting/tissue preparation method: staining after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 05 2010

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