PerCP Anti-CD11b antibody [M1/70] (ab237025)
![Flow Cytometry - PerCP Anti-CD11b antibody [M1/70] (ab237025)](https://www.abcam.com/ps/products/237/ab237025/Images/ab237025-314958-anti-cd11b-antibody-m1-70-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD11b antibody [M1/70] (ab237025)")
Key features and details
- PerCP Rat monoclonal [M1/70] to CD11b
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
Overview
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Product name
PerCP Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
PerCP Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus. (B10 mouse spleen cells enriched for T cells).
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Positive control
- Flow Cyt: Mouse bone marrow cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from an in vitro cell culture of a cell hybridoma. -
Clonality
Monoclonal -
Clone number
M1/70 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab237025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16409 Mouse
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Flow Cytometry - PerCP Anti-CD11b antibody [M1/70] (ab237025)
Flow cytometric analysis of mouse bone marrow cells stained for CD11b with ab237025 (filled histogram) and rat IgG2b PerCP isotype control (blue histogram).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab237025 has not yet been referenced specifically in any publications.
