PerCP Anti-CD14 antibody [47-3D6] (ab91146)
![Flow Cytometry - PerCP Anti-CD14 antibody [47-3D6] (ab91146)](https://www.abcam.com/ps/products/91/ab91146/Images/ab91146-200205-anti-cd14-antibody-47-3d6-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD14 antibody [47-3D6] (ab91146)")
Key features and details
- PerCP Mouse monoclonal [47-3D6] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Overview
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Product name
PerCP Anti-CD14 antibody [47-3D6]
See all CD14 primary antibodies -
Description
PerCP Mouse monoclonal [47-3D6] to CD14 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
ab91146 reacts with human monocytes and macrophages; weak reactions may occur with neutrophils. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human lung angiotensin converting enzyme.
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Positive control
- Human Normal peripheral blood
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
47-3D6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
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Flow Cytometry - PerCP Anti-CD14 antibody [47-3D6] (ab91146)This image is courtesy of an anonymous Abreview
ab91146 staining CD14 in the Human blood cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the undiluted primary antibody for 30 minutes at 4°C.
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![Flow Cytometry - PerCP Anti-CD14 antibody [47-3D6] (ab91146)](https://www.abcam.com/ps/products/91/ab91146/Images/CD14-Primary-antibodies-ab91146-3.jpg)
Flow Cytometry - PerCP Anti-CD14 antibody [47-3D6] (ab91146)ab91146, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.
References (2)
ab91146 has been referenced in 2 publications.
- Guo YH et al. Effects of ginsenoside Rg1-loaded alginate-chitosan microspheres on human bone marrow stromal cells. Biosci Rep 37:N/A (2017). PubMed: 28536312
- Hünniger K et al. A virtual infection model quantifies innate effector mechanisms and candida albicans immune escape in human blood. PLoS Comput Biol 10:e1003479 (2014). Flow Cyt ; Human . PubMed: 24586131
