PerCP Anti-CD14 antibody [MEM-15] (ab269327)

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Flow Cytometry - PerCP Anti-CD14 antibody [MEM-15] (ab269327)

    Key features and details

    • PerCP Mouse monoclonal [MEM-15] to CD14
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP. Ex: 482nm, Em: 675nm
    • Isotype: IgG1

    Overview

    • Product name

      PerCP Anti-CD14 antibody [MEM-15]
      See all CD14 primary antibodies

    • Description

      PerCP Mouse monoclonal [MEM-15] to CD14

    • Host species

      Mouse

    • Conjugation

      PerCP. Ex: 482nm, Em: 675nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Non human primates

    • Immunogen

      Full length protein corresponding to Human CD14. A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

    • Positive control

      • Flow cyt: Human peripheral blood cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269327 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Or 100 μL of whole blood.
    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Or 100 μL of whole blood.

    Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Flow Cytometry - PerCP Anti-CD14 antibody [MEM-15] (ab269327)
        Flow Cytometry - PerCP Anti-CD14 antibody [MEM-15] (ab269327)
        Flow cytometry analysis (surface staining) of human peripheral blood cells labeling CD14 with ab269327.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab269327? Please let us know so that we can cite the reference in this datasheet.

      ab269327 has not yet been referenced specifically in any publications.

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