PerCP Anti-CD20 antibody [2H7] (ab114860)
![Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)](https://www.abcam.com/ps/products/114/ab114860/Images/ab114860-287973-anti-cd20-antibody-2h7-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)")
Key features and details
- PerCP Mouse monoclonal [2H7] to CD20
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
Overview
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Product name
PerCP Anti-CD20 antibody [2H7]
See all CD20 primary antibodies -
Description
PerCP Mouse monoclonal [2H7] to CD20 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
Human tonsillar B cells.
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Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Primary antibody notes
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. -
Clonality
Monoclonal -
Clone number
2H7 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD20 antibody [2H7] (ab112461)
- Biotin Anti-CD20 antibody [2H7] (ab123487)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7], prediluted (ab155376)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7] (ab157321)
- PE/DyLight™ 594 Anti-CD20 antibody [2H7] (ab187593)
- PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)
- Alexa Fluor® 700 Anti-CD20 antibody [2H7] (ab269315)
- FITC Anti-CD20 antibody [2H7] (ab51792)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
(or 100 µl of whole blood). ab172890 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. (or 100 µl of whole blood). ab172890 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)
Surface staining of human peripheral blood with ab114860.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab114860 has been referenced in 2 publications.
- Deans JP et al. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem 273:344-8 (1998). PubMed: 9417086
- Deans JP et al. Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem 270:22632-8 (1995). PubMed: 7545683