PerCP Anti-CD20 antibody [2H7] (ab114860)
![Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)](https://www.abcam.com/ps/products/114/ab114860/Images/ab114860-287973-anti-cd20-antibody-2h7-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)")
Key features and details
- PerCP Mouse monoclonal [2H7] to CD20
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
Overview
-
Product name
PerCP Anti-CD20 antibody [2H7]
See all CD20 primary antibodies -
Description
PerCP Mouse monoclonal [2H7] to CD20 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
Human tonsillar B cells.
-
Positive control
- Flow Cyt: Human peripheral blood cells.
-
General notes
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Primary antibody notes
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. -
Clonality
Monoclonal -
Clone number
2H7 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
- PE Anti-CD20 antibody [2H7] (ab112461)
- Biotin Anti-CD20 antibody [2H7] (ab123487)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7], prediluted (ab155376)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7] (ab157321)
- PE/DyLight™ 594 Anti-CD20 antibody [2H7] (ab187593)
- PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)
- Alexa Fluor® 700 Anti-CD20 antibody [2H7] (ab269315)
- FITC Anti-CD20 antibody [2H7] (ab51792)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab114860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. (or 100 µl of whole blood). ab172890 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
-
Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
-
Flow Cytometry - PerCP Anti-CD20 antibody [2H7] (ab114860)
Surface staining of human peripheral blood with ab114860.
References (2)
ab114860 has been referenced in 2 publications.
- Deans JP et al. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem 273:344-8 (1998). PubMed: 9417086
- Deans JP et al. Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem 270:22632-8 (1995). PubMed: 7545683
