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    percp-cd28-antibody-cd282-ab88034.pdf

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Immunology Adaptive Immunity B Cells CD
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PerCP Anti-CD28 antibody [CD28.2] (ab88034)

  • Datasheet
  • SDS
Submit a review Submit a question References (7)

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Key features and details

  • PerCP Mouse monoclonal [CD28.2] to CD28
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG1

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Overview

  • Product name

    PerCP Anti-CD28 antibody [CD28.2]
    See all CD28 primary antibodies
  • Description

    PerCP Mouse monoclonal [CD28.2] to CD28
  • Host species

    Mouse
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm
  • Specificity

    Reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    DC28.1.3.3 hybridoma cells

  • Positive control

    • Human blood cells
  • General notes

    The purified antibody (>95% by SDS-PAGE) is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    0.2% BSA (high-grade, protease free)
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • Clonality

    Monoclonal
  • Clone number

    CD28.2
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
    • FITC Anti-CD28 antibody [CD28.2] (ab134472)
    • APC Anti-CD28 antibody [CD28.2], prediluted (ab155372)
    • PE/Cy7® Anti-CD28 antibody [CD28.2], prediluted (ab155387)
    • PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
    • FITC Anti-CD28 antibody [CD28.2] (ab234227)
    • PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
    • Biotin Anti-CD28 antibody [CD28.2] (ab267385)
    • APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
    • Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
  • Isotype control

    • PerCP Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab118658)
  • Recombinant Protein

    • Recombinant Human CD28 protein (His tag) (ab222975)

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P10747 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 940 Human
    • Omim: 186760 Human
    • SwissProt: P10747 Human
    • Unigene: 591629 Human
    • Alternative names

      • CD 28 antibody
      • CD28 antibody
      • CD28 antigen antibody
      • CD28 molecule antibody
      • CD28_HUMAN antibody
      • MGC138290 antibody
      • T cell antigen CD28 antibody
      • T cell specific surface glycoprotein antibody
      • T cell specific surface glycoprotein CD28 antibody
      • T-cell-specific surface glycoprotein CD28 antibody
      • TP44 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab88034? Please let us know so that we can cite the reference in this datasheet.

    ab88034 has been referenced in 7 publications.

    • Scharschmidt E  et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780
    • Jeong SH  et al. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol 78:6995-7003 (2004). PubMed: 15194776
    • Marti F  et al. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol 166:197-206 (2001). PubMed: 11123293
    • Galea-Lauri J  et al. Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol 163:62-70 (1999). PubMed: 10384100
    • Tazi A  et al. Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol 163:3511-5 (1999). PubMed: 10477625
    • Nunès J  et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol 5:311-5 (1993). PubMed: 8385476
    • Nunes J  et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J 293 ( Pt 3):835-42 (1993). PubMed: 8394695

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