PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
![Flow Cytometry - PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)](https://www.abcam.com/ps/products/106/ab106215/Images/ab106215-278859-anti-cd3-antibody-ucht1-prediluted-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)")
Key features and details
- PerCP Mouse monoclonal [UCHT1] to CD3, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Overview
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Product name
PerCP Anti-CD3 antibody [UCHT1], prediluted
See all CD3 primary antibodies -
Description
PerCP Mouse monoclonal [UCHT1] to CD3, prediluted -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
ab106215 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. It reacts with the epsilon chain of the CD3 complex. HLDA I; WS Code T 3 HLDA III; WS Code T 126 HLDA III; WS Code T 471 HLDA VI; WS Code T 6T-CD3.1 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
Human thymocytes followed by Sezary T cells
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Positive control
- Human blood cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 2.68% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
UCHT1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- Biotin Anti-CD3 antibody [UCHT1] (ab191112)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- FITC Anti-CD3 antibody [UCHT1] (ab34275)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)Flow Cytometry analysis of Human peripheral blood cells labeling CD3 with Anti-CD3 antibody [UCHT1], prediluted (PerCP) (ab106215).
References (7)
ab106215 has been referenced in 7 publications.
- Wang J et al. Calreticulin is an effective immunologic adjuvant to tumor-associated antigens. Exp Ther Med 14:3399-3406 (2017). PubMed: 29042925
- Siegers GM et al. Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med 204:2537-44 (2007). PubMed: 17923503
- Demedts IK et al. Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol 32:177-84 (2005). PubMed: 15576669
- Arnett KL et al. Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A 101:16268-73 (2004). PubMed: 15534202
- Torres PS et al. TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol 170:5947-55 (2003). PubMed: 12794121
- le Gouvello S et al. Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol 161:1113-22 (1998). PubMed: 9686569
- Garson JA et al. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature 298:375-7 (1982). PubMed: 6178042
