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  1. Link

    percp-cd4-antibody-mem-241-ab65951.pdf

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Immunology Adaptive Immunity T Cells CD
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PerCP Anti-CD4 antibody [MEM-241] (ab65951)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (3)

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Key features and details

  • PerCP Mouse monoclonal [MEM-241] to CD4
  • Suitable for: Flow Cyt
  • Reacts with: Human, Cynomolgus monkey
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG1

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Overview

  • Product name

    PerCP Anti-CD4 antibody [MEM-241]
    See all CD4 primary antibodies
  • Description

    PerCP Mouse monoclonal [MEM-241] to CD4
  • Host species

    Mouse
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Two N terminal domains of human CD4 fused to human IgG1 Fc

  • General notes

    The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-241
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • APC Anti-CD4 antibody [MEM-241] (ab18280)
    • FITC Anti-CD4 antibody [MEM-241] (ab18281)
    • PE Anti-CD4 antibody [MEM-241] (ab18282)
    • APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
    • PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
    • Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
    • Biotin Anti-CD4 antibody [MEM-241] (ab28069)
  • Isotype control

    • PerCP Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab118658)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab65951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (4)
Use at an assay dependent concentration.

ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use at an assay dependent concentration.

ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101864991 Cynomolgus monkey
    • Entrez Gene: 920 Human
    • Omim: 186940 Human
    • SwissProt: P79185 Cynomolgus monkey
    • SwissProt: P01730 Human
    • Unigene: 631659 Human
    • Alternative names

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab65951? Please let us know so that we can cite the reference in this datasheet.

    ab65951 has been referenced in 3 publications.

    • Chua KV  et al. Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90a Secretion, and Tumor Growth. Cells 9:N/A (2019). PubMed: 31905895
    • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . PubMed: 24388011
    • Bastien JP  et al. Photodepletion differentially affects CD4+ Tregs versus CD4+ effector T cells from patients with chronic graft-versus-host disease. Blood 116:4859-69 (2010). Flow Cyt ; Human . PubMed: 20798236

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 09 2013

    Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (PerCP)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (PerCP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (PerCP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Question

    Does this CD4 antibody cross-react with human IgG1? The immunogen for this product is listed as "two N terminal domains of human CD4 fused to human IgG1 Fc."

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2012

    Answer

    Thank you for contacting us. The lab has confirmed that this antibody is indeed specific to CD4. We will guarantee it not to react with any human IgG1 that may be present in your experiments. I hope this helps, please let me know if you need any additional information.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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