PerCP Anti-CD4 antibody [SK3] (ab210374)
Key features and details
- PerCP Mouse monoclonal [SK3] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Overview
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Product name
PerCP Anti-CD4 antibody [SK3]
See all CD4 primary antibodies -
Description
PerCP Mouse monoclonal [SK3] to CD4 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey -
Positive control
- Human peripheral blood lymphocytes
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General notes
ab210374 was conjugated under optimal conditions, with unreacted dye removed from the preparation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
ab210374 was purified from tissue culture supernatant via affinity chromatography. -
Clonality
Monoclonal -
Clone number
SK3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 5µl for 105-8 cells.
Final volume of approximately 100 µL. |
Notes |
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Flow Cyt
Use 5µl for 105-8 cells. Final volume of approximately 100 µL. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 101864991 Cynomolgus monkey
- Entrez Gene: 920 Human
- Entrez Gene: 713807 Rhesus monkey
- Omim: 186940 Human
- SwissProt: P79185 Cynomolgus monkey
- SwissProt: P01730 Human
- SwissProt: P16003 Rhesus monkey
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210374 has not yet been referenced specifically in any publications.