PerCP Anti-CD4 antibody [SK3] (ab210374)

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Flow Cytometry - PerCP Anti-CD4 antibody [SK3] (ab210374)

    Key features and details

    • PerCP Mouse monoclonal [SK3] to CD4
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP. Ex: 482nm, Em: 675nm
    • Isotype: IgG1

    Overview

    • Product name

      PerCP Anti-CD4 antibody [SK3]
      See all CD4 primary antibodies

    • Description

      PerCP Mouse monoclonal [SK3] to CD4

    • Host species

      Mouse

    • Conjugation

      PerCP. Ex: 482nm, Em: 675nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Cynomolgus monkey, Rhesus monkey

    • Positive control

      • Human peripheral blood lymphocytes

    • General notes

      ab210374 was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab210374 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    Final volume of approximately 100 µL.
    Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    Final volume of approximately 100 µL.

    Target

    • Function

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

    • Cellular localization

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Images

      • Flow Cytometry - PerCP Anti-CD4 antibody [SK3] (ab210374)
        Flow Cytometry - PerCP Anti-CD4 antibody [SK3] (ab210374)

        Human peripheral blood lymphocytes were stained with 5 uL (0.06 ug) ab210374 (solid line) or 0.06 ug PerCP Mouse IgG1 isotype control (dashed line).

      References (0)

      Publishing research using ab210374? Please let us know so that we can cite the reference in this datasheet.

      ab210374 has not yet been referenced specifically in any publications.

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