Key features and details
- PerCP Rat monoclonal [FA-11] to CD68
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2a
Product namePerCP Anti-CD68 antibody [FA-11]
See all CD68 primary antibodies
DescriptionPerCP Rat monoclonal [FA-11] to CD68
ConjugationPerCP. Ex: 482nm, Em: 675nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse CD68.
Database link: P31996
- Flow Cyt: Raw264.7 cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
- Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
- Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
- HRP Anti-CD68 antibody [FA-11] (ab216526)
- PE Anti-CD68 antibody [FA-11] (ab216701)
- APC Anti-CD68 antibody [FA-11] (ab221251)
- Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
- Anti-CD68 antibody [FA-11] (ab53444)
Our Abpromise guarantee covers the use of ab220509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Overlay histogram showing Raw264.7 cells stained with ab220509 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220509, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab220509 has not yet been referenced specifically in any publications.