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  1. Link

    percp-cd8-antibody-mem-31-ab65949.pdf

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Immunology Adaptive Immunity T Cells CD
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PerCP Anti-CD8 antibody [MEM-31] (ab65949)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Key features and details

  • PerCP Mouse monoclonal [MEM-31] to CD8
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG2a

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Conjugation
Product image
PerCP Conjugation Kit - Lightning-Link® (ab102907)

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Overview

  • Product name

    PerCP Anti-CD8 antibody [MEM-31]
    See all CD8 primary antibodies
  • Description

    PerCP Mouse monoclonal [MEM-31] to CD8
  • Host species

    Mouse
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm
  • Specificity

    ab65949 recognizes a conformationally-dependent epitope of CD8. The antibody does not react with formaldehyde-fixed cells and is negative in Western Blotting.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human):Crude thymus membrane fraction.

  • Positive control

    • human blood
  • General notes

    Conjugated with Peridinin-chlorophyll-protein complex (PerCP)

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    >95% by SDS-PAGE
  • Clonality

    Monoclonal
  • Clone number

    MEM-31
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • PerCP/Cy5.5® Anti-CD8 antibody [MEM-31] (ab157306)
    • APC/Cy7® Anti-CD8 antibody [MEM-31] (ab233300)
    • PE/Cy7® Anti-CD8 antibody [MEM-31] (ab239316)
    • APC Anti-CD8 antibody [MEM-31] (ab26004)
    • Biotin Anti-CD8 antibody [MEM-31] (ab28090)
  • Isotype control

    • PerCP Mouse IgG2a [MOPC-173] - Isotype Control (ab209397)

Applications

  • Application notes
    Flow Cyt: Use Neat: 10µl for 106 cells, or 100µl whole blood.


    Is unsuitable for WB.
    Is unsuitable for applications using formaldehyde fixed cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P01732 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 925 Human
      • Omim: 186910 Human
      • SwissProt: P01732 Human
      • Unigene: 85258 Human
      • Form

        CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
      • Alternative names

        • CD8 antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8a antigen antibody
        • CD8a molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        see all

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab65949? Please let us know so that we can cite the reference in this datasheet.

      ab65949 has been referenced in 2 publications.

      • Abu N  et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 18:31 (2018). PubMed: 29374471
      • Linnebacher M  et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). PubMed: 20339516

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-CD8 antibody [MEM-31] (PerCP)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Permeabilization
      No
      Sample
      Human Cell (whole blood)
      Specification
      whole blood
      Gating Strategy
      lymphocytes
      Preparation
      Cell harvesting/tissue preparation method: Ficoll-Hypaque
      Sample buffer: PBS
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 19 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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