PerCP Anti-CD8 antibody [MEM-31] (ab65949)
Key features and details
- PerCP Mouse monoclonal [MEM-31] to CD8
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2a
Overview
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Product name
PerCP Anti-CD8 antibody [MEM-31]
See all CD8 primary antibodies -
Description
PerCP Mouse monoclonal [MEM-31] to CD8 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
ab65949 recognizes a conformationally-dependent epitope of CD8. The antibody does not react with formaldehyde-fixed cells and is negative in Western Blotting. -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human):Crude thymus membrane fraction.
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Positive control
- human blood
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General notes
Conjugated with Peridinin-chlorophyll-protein complex (PerCP)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
>95% by SDS-PAGE -
Clonality
Monoclonal -
Clone number
MEM-31 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Application notes
Flow Cyt: Use Neat: 10µl for 106 cells, or 100µl whole blood.
Is unsuitable for WB.
Is unsuitable for applications using formaldehyde fixed cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for WB.
Is unsuitable for applications using formaldehyde fixed cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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Form
CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation. -
Alternative names
- CD8 antibody
- CD8 antigen, alpha polypeptide (p32) antibody
- CD8a antibody
see all
References (2)
ab65949 has been referenced in 2 publications.
- Abu N et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 18:31 (2018). PubMed: 29374471
- Linnebacher M et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). PubMed: 20339516
