PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
Key features and details
- PerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]
See all CD90 / Thy1 primary antibodies -
Description
PerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Horse -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: PC12 (NGF differentiated) cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-7 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
The cellular localisation of this product has been verified in ICC/IF.
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Notes |
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Flow Cyt
1/100. The cellular localisation of this product has been verified in ICC/IF.
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Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24832 Rat
- SwissProt: P01830 Rat
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Flow cytometry overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab216354) (1x 106 in 100μl at 5μg/ml (1/100)) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 690/50 bandpass filter.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216354 has not yet been referenced specifically in any publications.