Key features and details
- PerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Product namePerCP Anti-CD90 / Thy1 antibody [MRC OX-7]
See all CD90 / Thy1 primary antibodies
DescriptionPerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
ConjugationPerCP. Ex: 482nm, Em: 675nm
SpecificityRecognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
Predicted to work with: Horse
Full length protein corresponding to Rat CD90/ Thy1 aa 1 to the C-terminus.
Database link: P01830
- Flow Cyt: PC12 (NGF differentiated)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Clone numberMRC OX-7
Light chain typekappa
Our Abpromise guarantee covers the use of ab216354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionMay play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
Overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216354, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
This antibody gave a positive signal in PC12 (NGF differentiated) cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab216354 has not yet been referenced specifically in any publications.