PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
![Flow Cytometry - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)](https://www.abcam.com/ps/products/216/ab216354/Images/ab216354-279938-anti-cd90-thy1-antibody-mrc-ox-7-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)")
Key features and details
- PerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Overview
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Product name
PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]
See all CD90 / Thy1 primary antibodies -
Description
PerCP Mouse monoclonal [MRC OX-7] to CD90 / Thy1 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. As Thy 1 is monomorphic in rat it will react with all rat strains. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Horse
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Immunogen
Full length protein corresponding to Rat CD90/ Thy1 aa 1 to the C-terminus.
Database link: P01830 -
Positive control
- Flow Cyt: PC12 (NGF differentiated)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-7 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab216354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24832 Rat
- SwissProt: P01830 Rat
- Unigene: 108198 Rat
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Flow Cytometry - PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
Overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216354, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
This antibody gave a positive signal in PC12 (NGF differentiated) cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
References (0)
ab216354 has not yet been referenced specifically in any publications.
