PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)

Flow cytometry overlay histogram showing PC12 (NGF differentiated) cells stained with ab216354 (red line). The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab216354) (1x 10⁶ in 100μl at 5μg/ml (1/100)) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 690/50 bandpass filter.