PerCP Conjugation Kit - Lightning-Link® (ab102907)

Submit a review Q&A (3)References (6)
Conjugation - PerCP Conjugation Kit (ab102907)

    Overview

    • Product name

      PerCP Conjugation Kit - Lightning-Link®

    • Product overview

      PerCP Conjugation Kit / PerCP Labeling Kit ab102907 uses a simple and quick process for PerCP labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


      To conjugate an antibody to PerCP using this kit:
      - add modifier to antibody and incubate for 3 hrs
      - add quencher and incubate for 30 mins
      The PerCP conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


      Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to PerCP.


      Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

    • Notes

      This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® PerCP Labeling Kit. 718-0015 is the same as the 1 mg size. 718-0010 is the same as the 3 x 100 ug size. 718-0030 is the same as the 3 x 10 ug size. 718-0005 is the same as the 100 µg size.

      Amount and volume of antibody for conjugation to PerCP

       Kit size Recommended maximum
      amount of antibody             		 Maximum antibody 
      volume1
      3 x 10 µg 3 x 10 µg 3 x 10 µL
      100 µg 1 x 100 µg  1 x 100 µL
      3 x 100 µg 3 x 100 µg  3 x 100 µL
      1 mg 1 x 1 mg 1 x 1 mL

      1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

       

      Buffer Requirements for Conjugation

      Buffer should be pH 6.5-8.5.

      Compatible buffer constituents 
      If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

      50mM / 0.6% Tris1 0.1% BSA 50% glycerol
      0.1% sodium azide PBS Potassium phosphate
      Sodium chloride HEPES Sucrose
      Sodium citrate EDTA Trehalose

      1 Tris buffered saline is almost always ≤ 50 mM / 0.6%

      Incompatible buffer constituents

      Thiomerosal Proclin Glycine
      Arginine Glutathione DTT

      If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

      Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture 

      Storing and handling conjugation kits

      Lyophilized Lightning-Link® components are hygroscopic.

      Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg
      ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
      ab274141 - PerCP mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg
      ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl

    • Research areas

    • Alternative names

      • Peridinin chlorophyll protein complex

    Images

    • Conjugation - PerCP Conjugation Kit (ab102907)
      Conjugation - PerCP Conjugation Kit (ab102907)

      This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

    References (6)

    Publishing research using ab102907? Please let us know so that we can cite the reference in this datasheet.

    ab102907 has been referenced in 6 publications.

    • Nagy C  et al. Single-nucleus transcriptomics of the prefrontal cortex in major depressive disorder implicates oligodendrocyte precursor cells and excitatory neurons. Nat Neurosci 23:771-781 (2020). PubMed: 32341540
    • Bayik D  et al. Hepatobiliary malignancies have distinct peripheral myeloid-derived suppressor cell signatures and tumor myeloid cell profiles. Sci Rep 10:18848 (2020). PubMed: 33139767
    • Keeley EC  et al. Circulating fibrocytes as predictors of adverse events in unstable angina. Transl Res 172:73-83.e1 (2016). PubMed: 27012475
    • Bok S  et al. In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy. Biomed Opt Express 6:3303-12 (2015). PubMed: 26417502
    • Rodgers JM  et al. IL-17A activates ERK1/2 and enhances differentiation of oligodendrocyte progenitor cells. Glia 63:768-79 (2015). PubMed: 25557204
    • Yasmin AR  et al. In vitro characterization of chicken bone marrow-derived dendritic cells following infection with very virulent infectious bursal disease virus. Avian Pathol 44:452-62 (2015). PubMed: 26305169

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Has 100 ug of a protein in 250 uL. How should protocol be modified? The first step says to add 10 uL of modifier to 100 uL of antibody- should 25 uL be added since 250 uL of antibody is used?

    Read More
    Answer

    Thank you for your call yesterday and for your patience while I have been in touch with the scientists in the lab regarding your enquiry.

    My colleagues have pointed out that the protein should be concentrated for the best results. The entire 250 uL should not be added to the single 100 ug vial of conjugate even though the protein is less concentrated. The conjugation will not work with 250 uL of sample volume. If the protein can not be concentrated, you can try with the dilute protein but the 250 uL will need to be split over the multiple vials of conjugate, so that no more than 100 uL will be added per vial. This should give some conjugated product but please note that the results will not be optimal.

    I hope this information will be useful, but please let me know if you have any further questions or need anything else regarding these kits, and I will be happy to help. Have a nice day.

    Read More

    Question

    Hello, I have another question concerning the PerCP/Cya5.5 reagent I got in the labeling kit. Could you tell me the amount (either estimation or measured) of Cya5.5 per PerCP molecule ? Thank you and best regards,

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately, I am unable to tell you the number of Cy5.5 molecules. The amount of Cy5.5 per PerCP in the PerCP/Cy5.5 conjugation kit has been optimised to give excellent performance.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I ordered kits mentionned below. I would like to know if you have any data concerning the extinction coefficient of PerCP and PerCP-Cy55 at its optimal absorption wavelength. Thank you and best regards,

    Read More
    Answer

    Thank you for contacting Abcam. Here are the details you requested. Ab102907 (PerCP ): Abs max=482nm, Emission Max= 677nm , Extinction coefficient (cm-1M-1)= 380,000 Ab102911(PerCP/Cy5.5): Abs max=482nm, Emission Max= 700nm , Extinction coefficient (cm-1M-1)= unknown I hope this information is helpful. Please let me know if you have any questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com