For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    percp-conjugation-kit-lightning-linkreg-ab102907.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Epitope Tags Conjugates
Share by email

PerCP Conjugation Kit - Lightning-Link® (ab102907)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Conjugation - PerCP Conjugation Kit  (ab102907)

    You may also be interested in

    Conjugation
    Product image
    Antibody Concentration And Clean-Up Kit (ab102778)
    Conjugation
    Product image
    APC/Cy7® Conjugation Kit - Lightning-Link® (ab102859)
    Conjugation
    Product image
    BSA Removal Kit (ab173231)

    View more associated products

    Overview

    • Product name

      PerCP Conjugation Kit - Lightning-Link®
    • Product overview

      PerCP Conjugation Kit / PerCP Labeling Kit ab102907 uses a simple and quick process for PerCP labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


      To conjugate an antibody to PerCP using this kit:
      - add modifier to antibody and incubate for 3 hrs
      - add quencher and incubate for 30 mins
      The PerCP conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


      Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to PerCP.


      Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

    • Notes

      This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® PerCP Labeling Kit. 718-0015 is the same as the 1 mg size. 718-0010 is the same as the 3 x 100 ug size. 718-0030 is the same as the 3 x 10 ug size. 718-0005 is the same as the 100 µg size.

      Amount and volume of antibody for conjugation to PerCP

       Kit size Recommended maximum
      amount of antibody
       
      Maximum antibody 
      volume1
      3 x 10 µg 3 x 10 µg 3 x 10 µL
      100 µg 1 x 100 µg  1 x 100 µL
      3 x 100 µg 3 x 100 µg  3 x 100 µL
      1 mg 1 x 1 mg 1 x 1 mL

      1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

       

      Buffer Requirements for Conjugation

      Buffer should be pH 6.5-8.5.

      Compatible buffer constituents 
      If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

      50mM / 0.6% Tris1 0.1% BSA 50% glycerol
      0.1% sodium azide PBS Potassium phosphate
      Sodium chloride HEPES Sucrose
      Sodium citrate EDTA Trehalose

      1 Tris buffered saline is almost always ≤ 50 mM / 0.6%

      Incompatible buffer constituents

      Thiomerosal Proclin Glycine
      Arginine Glutathione DTT

      If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

      Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture 

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg
      ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
      ab274141 - PerCP mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg
      ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Conjugates
      • Kits/ Lysates/ Other
      • Kits
      • Conjugation Kits
      • PerCP
    • Alternative names

      • Peridinin chlorophyll protein complex

    Associated products

    • Related Products

      • Antibody Concentration And Clean-Up Kit (ab102778)
      • Antibody Purification Kit (Protein A) (ab102784)

    Images

    • Conjugation - PerCP Conjugation Kit  (ab102907)
      Conjugation - PerCP Conjugation Kit (ab102907)

      This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

    Protocols

    • Protocol Booklet - Chinese Version
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab102907? Please let us know so that we can cite the reference in this datasheet.

    ab102907 has been referenced in 1 publication.

    • Bok S  et al. In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy. Biomed Opt Express 6:3303-12 (2015). PubMed: 26417502

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Has 100 ug of a protein in 250 uL. How should protocol be modified? The first step says to add 10 uL of modifier to 100 uL of antibody- should 25 uL be added since 250 uL of antibody is used?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2012

    Answer

    Thank you for your call yesterday and for your patience while I have been in touch with the scientists in the lab regarding your enquiry.

    My colleagues have pointed out that the protein should be concentrated for the best results. The entire 250 uL should not be added to the single 100 ug vial of conjugate even though the protein is less concentrated. The conjugation will not work with 250 uL of sample volume. If the protein can not be concentrated, you can try with the dilute protein but the 250 uL will need to be split over the multiple vials of conjugate, so that no more than 100 uL will be added per vial. This should give some conjugated product but please note that the results will not be optimal.

    I hope this information will be useful, but please let me know if you have any further questions or need anything else regarding these kits, and I will be happy to help. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Jun 14 2012

    Question

    Hello, I have another question concerning the PerCP/Cya5.5 reagent I got in the labeling kit. Could you tell me the amount (either estimation or measured) of Cya5.5 per PerCP molecule ? Thank you and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 09 2012

    Answer

    Thank you for contacting us.

    Unfortunately, I am unable to tell you the number of Cy5.5 molecules. The amount of Cy5.5 per PerCP in the PerCP/Cy5.5 conjugation kit has been optimised to give excellent performance.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 09 2012

    Question

    I ordered kits mentionned below. I would like to know if you have any data concerning the extinction coefficient of PerCP and PerCP-Cy55 at its optimal absorption wavelength. Thank you and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 10 2012

    Answer

    Thank you for contacting Abcam. Here are the details you requested. Ab102907 (PerCP ): Abs max=482nm, Emission Max= 677nm , Extinction coefficient (cm-1M-1)= 380,000 Ab102911(PerCP/Cy5.5): Abs max=482nm, Emission Max= 700nm , Extinction coefficient (cm-1M-1)= unknown I hope this information is helpful. Please let me know if you have any questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.