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  1. Link

    percp-f480-antibody-bm8-ab237026.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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PerCP Anti-F4/80 antibody [BM8] (ab237026)

  • Datasheet
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Flow Cytometry - PerCP Anti-F4/80 antibody [BM8] (ab237026)

    Key features and details

    • PerCP Rat monoclonal [BM8] to F4/80
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PerCP. Ex: 482nm, Em: 675nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Biotin FITC Unconjugated

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    Overview

    • Product name

      PerCP Anti-F4/80 antibody [BM8]
      See all F4/80 primary antibodies
    • Description

      PerCP Rat monoclonal [BM8] to F4/80
    • Host species

      Rat
    • Conjugation

      PerCP. Ex: 482nm, Em: 675nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse F4/80. (Murine macrophages).

    • Positive control

      • Flow Cyt: Thioglycolate-elicited Balb/c mouse peritoneal macrophages.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      Purified from an in vitro cell culture of a cell hybridoma.
    • Clonality

      Monoclonal
    • Clone number

      BM8
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types

    Associated products

    • Alternative Versions

      • Biotin Anti-F4/80 antibody [BM8] (ab15694)
      • Anti-F4/80 antibody [BM8] (ab16911)
      • FITC Anti-F4/80 antibody [BM8] (ab60343)
    • Recombinant Protein

      • Recombinant Human EMR1/ADGRE1 protein (ab131740)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab237026 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.
    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    Target

    • Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
    • Tissue specificity

      Expression is restricted to eosinophils.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
      Contains 6 EGF-like domains.
      Contains 1 GPS domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q14246 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 13733 Mouse
      • SwissProt: Q61549 Mouse
      • Unigene: 2254 Mouse
      • Alternative names

        • ADGRE1 antibody
        • Adhesion G protein coupled receptor E1 antibody
        • Adhesion G protein-coupled receptor E1 antibody
        • AGRE1_HUMAN antibody
        • Cell surface glycoprotein EMR1 antibody
        • Cell surface glycoprotein F4/80 antibody
        • DD7A5 7 antibody
        • Egf like module containing mucin like hormone receptor like 1 antibody
        • Egf like module containing mucin like hormone receptor like sequence 1 antibody
        • EGF like module receptor 1 antibody
        • EGF TM7 antibody
        • EGF-like module receptor 1 antibody
        • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
        • EGFTM7 antibody
        • EMR 1 antibody
        • EMR1 antibody
        • EMR1 hormone receptor antibody
        • Gpf480 antibody
        • Ly71 antibody
        • Lymphocyte antigen 71 antibody
        • TM7LN3 antibody
        see all

      Images

      • Flow Cytometry - PerCP Anti-F4/80 antibody [BM8] (ab237026)
        Flow Cytometry - PerCP Anti-F4/80 antibody [BM8] (ab237026)

        Flow cytometric analysis of thioglycolate-elicited Balb/c mouse peritoneal macrophages stained for F4/80 with ab237026 (filled histogram) and PerCP rat IgG2a isotype control (blue histogram).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab237026? Please let us know so that we can cite the reference in this datasheet.

      ab237026 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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