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Immunology Adaptive Immunity MHC Class II
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PerCP Anti-HLA-DR antibody [MEM-12] (ab239298)

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Flow Cytometry - PerCP Anti-HLA-DR antibody [MEM-12] (ab239298)

    Key features and details

    • PerCP Mouse monoclonal [MEM-12] to HLA-DR
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP. Ex: 482nm, Em: 675nm
    • Isotype: IgG1

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    Overview

    • Product name

      PerCP Anti-HLA-DR antibody [MEM-12]
      See all HLA-DR primary antibodies
    • Description

      PerCP Mouse monoclonal [MEM-12] to HLA-DR
    • Host species

      Mouse
    • Conjugation

      PerCP. Ex: 482nm, Em: 675nm
    • Specificity

      ab239298 recognizes a common epitope on human HLA-DR which is dependent on the association of alpha and beta chains.
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Tissue, cells or virus corresponding to Human HLA-DR. (Thymocyte membrane).

    • Positive control

      • Flow Cyt: Human peripheral blood cells.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
    • Clonality

      Monoclonal
    • Clone number

      MEM-12
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • MHC
      • Class II

    Associated products

    • Alternative Versions

      • APC Anti-HLA-DR antibody [MEM-12] (ab239283)
      • Anti-HLA-DR antibody [MEM-12] (ab28322)
      • FITC Anti-HLA-DR antibody [MEM-12] (ab28323)
      • PE Anti-HLA-DR antibody [MEM-12] (ab28327)
    • Isotype control

      • PerCP Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab118658)
    • Recombinant Protein

      • Recombinant Human HLA-DR protein (ab177661)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab239298 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    (or 100 µl of whole blood)

    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    (or 100 µl of whole blood)

    Target

    • Function

      Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • Sequence similarities

      Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Target information above from: UniProt accession P01903 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3122 Human
      • Omim: 142860 Human
      • SwissProt: P01903 Human
      • Unigene: 520048 Human
      • Alternative names

        • DASS-397D15.1 antibody
        • DR alpha chain antibody
        • DR alpha chain precursor antibody
        • DRA_HUMAN antibody
        • DRB1 antibody
        • DRB4 antibody
        • FLJ51114 antibody
        • Histocompatibility antigen HLA DR alpha antibody
        • Histocompatibility antigen HLA-DR alpha antibody
        • HLA class II histocompatibility antigen antibody
        • HLA class II histocompatibility antigen DR alpha chain antibody
        • HLA DR1B antibody
        • HLA DR3B antibody
        • HLA DRA antibody
        • HLA DRA1 antibody
        • HLA DRB1 antibody
        • HLA DRB3 antibody
        • HLA DRB4 antibody
        • HLA DRB5 antibody
        • HLA-DR histocompatibility type antibody
        • HLA-DRA antibody
        • HLADR4B antibody
        • HLADRA1 antibody
        • HLADRB antibody
        • Major histocompatibility complex class II DR alpha antibody
        • Major histocompatibility complex class II DR beta 1 antibody
        • Major histocompatibility complex class II DR beta 3 antibody
        • Major histocompatibility complex class II DR beta 4 antibody
        • Major histocompatibility complex class II DR beta 5 antibody
        • MGC117330 antibody
        • MHC cell surface glycoprotein antibody
        • MHC class II antigen DRA antibody
        • MHC II antibody
        • MLRW antibody
        • OTTHUMP00000029406 antibody
        • OTTHUMP00000029407 antibody
        see all

      Images

      • Flow Cytometry - PerCP Anti-HLA-DR antibody [MEM-12] (ab239298)
        Flow Cytometry - PerCP Anti-HLA-DR antibody [MEM-12] (ab239298)

        Flow cytometric analysis of human peripheral blood cells labeling HLA-DR with ab239298. Surface staining. Gated on leukocytes.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab239298? Please let us know so that we can cite the reference in this datasheet.

      ab239298 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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