Recombinant PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
![Flow Cytometry (Intracellular) - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)](https://www.abcam.com/ps/products/220/ab220518/Images/ab220518-295202-anti-il-10-antibody-epr1114-percp-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PerCP Rabbit monoclonal [EPR1114] to IL-10
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
Related conjugates and formulations
Overview
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Product name
PerCP Anti-IL-10 antibody [EPR1114]
See all IL-10 primary antibodies -
Description
PerCP Rabbit monoclonal [EPR1114] to IL-10 -
Host species
Rabbit -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab169704) -
Positive control
- Flow Cyt (intra): THP-1 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1114 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Flow Cytometry (Intracellular) - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
Overlay histogram showing THP-1 cells stained with ab220518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220518, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
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![PerCP Anti-IL-10 antibody [EPR1114] (ab220518)](https://www.abcam.com/ps/products/220/ab220518/Images/ab220518-3-benefits-of-recombinant-antibodies.png)
PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab220518 has not yet been referenced specifically in any publications.
