Recombinant PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
![Flow Cytometry - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)](https://www.abcam.com/ps/products/220/ab220518/Images/ab220518-295202-anti-il-10-antibody-epr1114-percp-flow-cytometry.jpg "Flow Cytometry - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PerCP Rabbit monoclonal [EPR1114] to IL-10
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
Overview
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Product name
PerCP Anti-IL-10 antibody [EPR1114]
See all IL-10 primary antibodies -
Description
PerCP Rabbit monoclonal [EPR1114] to IL-10 -
Host species
Rabbit -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- Flow Cyt: THP-1 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR1114 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab220518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
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Alternative names
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
Images
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Flow Cytometry - PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
Overlay histogram showing THP-1 cells stained with ab220518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab220518, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
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![PerCP Anti-IL-10 antibody [EPR1114] (ab220518)](https://www.abcam.com/ps/products/220/ab220518/Images/ab220518-3-benefits-of-recombinant-antibodies.png)
PerCP Anti-IL-10 antibody [EPR1114] (ab220518)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab220518 has not yet been referenced specifically in any publications.
