PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)](https://www.abcam.com/ps/products/234/ab234265/Images/ab234265-320262-anti-cd105-antibody-mem-229-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [MEM-229] to CD105
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2a
Overview
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Product name
PerCP/Cy5.5® Anti-CD105 antibody [MEM-229]
See all CD105 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [MEM-229] to CD105 -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
Does not react with:
Horse, Dog -
Immunogen
Tissue, cells or virus corresponding to Human CD105. Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
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Positive control
- Flow cyt: HUVEC cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
MEM-229 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab234265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. (or 100 μL whole blood) |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)Surface staining of CD105 on Huvec cells with ab234265 at 1/25 dilution
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![Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)](https://www.abcam.com/ps/products/234/ab234265/Images/ab234265-308339-anti-cd105-antibody-mem-229-percp-cy55-flow-cytometry.jpg)
Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (ab234265)Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) cells, labeling CD105 with ab234265 at 4 μL per 106 cells (red) compared to a negative control (blue). Gated on HUVEC cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab234265 has been referenced in 1 publication.
- Yang C et al. Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica. Int J Mol Med 43:1395-1405 (2019). PubMed: 30628649
