PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

Submit a review Submit a question
Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

    Key features and details

    • PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD11c antibody [N418]
      See all CD11c primary antibodies

    • Description

      PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c

    • Host species

      Armenian hamster

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Specificity

      N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Preparation of spleen dendritic cells (Mouse)

    • Positive control

      • Splenocytes.

    • General notes

      Excitation wavelength: 488 nm. Emission wavelength: 690 nm.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab111469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent dilution. This can be used at less than or equal to 0.25µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

    Target

    • Function

      Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

    • Tissue specificity

      Predominantly expressed in monocytes and granulocytes.

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.

    • Domain

      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 95 alpha chain antibody
        • 95 antibody
        • CD 11c antibody
        • CD11 antigen like family member C antibody
        • CD11 antigen-like family member C antibody
        • CD11c antibody
        • CD11c antigen antibody
        • Complement component 3 receptor 4 subunit antibody
        • CR4 antibody
        • Integrin alpha X antibody
        • Integrin alpha X chain antibody
        • Integrin alpha-X antibody
        • Integrin aX antibody
        • Integrin subunit alpha X antibody
        • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
        • integrin, alpha X (complement component 3 receptor 4 subunit antibody
        • ITAX_HUMAN antibody
        • ITGAX antibody
        • LEU M5 alpha subunit antibody
        • Leu M5 antibody
        • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
        • Leukocyte adhesion glycoprotein p150 antibody
        • Leukocyte adhesion receptor p150 95 antibody
        • Leukocyte adhesion receptor p150 antibody
        • Leukocyte surface antigen p150 95 alpha subunit antibody
        • Leukocyte surface antigen p150 alpha subunit antibody
        • Myeloid membrane antigen alpha subunit antibody
        • p150 95 integrin alpha chain antibody
        • p150 antibody
        • p150/95 antibody
        • SLEB6 antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

        Flow cytometry analysis of C57BI/6 splenocytes stained with ab111469 at 0.25μg (Left panel). Right panel showing analysis of C57BI/6 splenocytes stain with 0.25μg PerCP-Cy5.5 Anti-Mouse CD11c 

      References (0)

      Publishing research using ab111469? Please let us know so that we can cite the reference in this datasheet.

      ab111469 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab111469.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com