PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)](https://www.abcam.com/ps/products/111/ab111469/Images/ab111469-279771-anti-cd11c-antibody-n418-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)")
Key features and details
- PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
PerCP/Cy5.5® Anti-CD11c antibody [N418]
See all CD11c primary antibodies -
Description
PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c -
Host species
Armenian hamster -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Specificity
N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD11c. Mouse spleen dendritic cells
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Positive control
- Splenocytes.
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General notes
Excitation wavelength: 488 nm. Emission wavelength: 690 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
N418 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD11c antibody [N418] - Low endotoxin, Azide free (ab185783)
- APC Anti-CD11c antibody [N418] (ab210306)
- APC/Cy7® Anti-CD11c antibody [N418] (ab210307)
- FITC Anti-CD11c antibody [N418] (ab210308)
- PE Anti-CD11c antibody [N418] (ab210309)
- PE/Cy7® Anti-CD11c antibody [N418] (ab210310)
- Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)
- Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
- violetFluor™ 450 Anti-CD11c antibody [N418] (ab242269)
- Anti-CD11c antibody [N418] (ab33483)
- Alexa Fluor® 488 Anti-CD11c antibody [N418] (ab33503)
- Biotin Anti-CD11c antibody [N418] (ab95765)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent dilution. This can be used at less than or equal to 0.25µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should
be determined empirically but can range from 10^5 to 10^8 cells/test.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent dilution. This can be used at less than or equal to 0.25µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. |
Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16411 Mouse
- SwissProt: Q9QXH4 Mouse
- Unigene: 22378 Mouse
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)
Flow cytometry analysis of C57BI/6 splenocytes stained with ab111469 at 0.25μg (Left panel). Right panel showing analysis of C57BI/6 splenocytes stain with 0.25μg PerCP-Cy5.5 Anti-Mouse CD11c
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111469 has not yet been referenced specifically in any publications.