PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

    Key features and details

    • PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD11c antibody [N418]
      See all CD11c primary antibodies

    • Description

      PerCP/Cy5.5® Armenian hamster monoclonal [N418] to CD11c

    • Host species

      Armenian hamster

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Specificity

      N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Preparation of spleen dendritic cells (Mouse)

    • Positive control

      • Splenocytes.

    • General notes

      Excitation wavelength: 488 nm. Emission wavelength: 690 nm.

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab111469 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent dilution. This can be used at less than or equal to 0.25µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
    Notes
    Flow Cyt
    Use at an assay dependent dilution. This can be used at less than or equal to 0.25µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

    Target

    • Function

      Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

    • Tissue specificity

      Predominantly expressed in monocytes and granulocytes.

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.

    • Domain

      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 95 alpha chain antibody
        • 95 antibody
        • CD 11c antibody
        • CD11 antigen like family member C antibody
        • CD11 antigen-like family member C antibody
        • CD11c antibody
        • CD11c antigen antibody
        • Complement component 3 receptor 4 subunit antibody
        • CR4 antibody
        • Integrin alpha X antibody
        • Integrin alpha X chain antibody
        • Integrin alpha-X antibody
        • Integrin aX antibody
        • Integrin subunit alpha X antibody
        • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
        • integrin, alpha X (complement component 3 receptor 4 subunit antibody
        • ITAX_HUMAN antibody
        • ITGAX antibody
        • LEU M5 alpha subunit antibody
        • Leu M5 antibody
        • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
        • Leukocyte adhesion glycoprotein p150 antibody
        • Leukocyte adhesion receptor p150 95 antibody
        • Leukocyte adhesion receptor p150 antibody
        • Leukocyte surface antigen p150 95 alpha subunit antibody
        • Leukocyte surface antigen p150 alpha subunit antibody
        • Myeloid membrane antigen alpha subunit antibody
        • p150 95 integrin alpha chain antibody
        • p150 antibody
        • p150/95 antibody
        • SLEB6 antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)

        Flow cytometry analysis of C57BI/6 splenocytes stained with ab111469 at 0.25μg (Left panel). Right panel showing analysis of C57BI/6 splenocytes stain with 0.25μg PerCP-Cy5.5 Anti-Mouse CD11c 

      References (0)

      Publishing research using ab111469? Please let us know so that we can cite the reference in this datasheet.

      ab111469 has not yet been referenced specifically in any publications.

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