PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)](https://www.abcam.com/ps/products/234/ab234266/Images/ab234266-308343-anti-cd15-antibody-mem-158-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgM
Overview
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Product name
PerCP/Cy5.5® Anti-CD15 antibody [MEM-158]
See all CD15 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [MEM-158] to CD15 -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Tissue, cells or virus corresponding to Human CD15. Human granulocytes.
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Positive control
- Flow cyt: Human peripheral blood cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
MEM-158 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab234266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. (or 100 μL whole blood) |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)
Flow cytometric analysis of surface stained human peripheral blood cells, labeling CD15 with ab234266 at 4 μL per 106 cells. Gated on leukocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab234266 has not yet been referenced specifically in any publications.
