PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [581] to CD34, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human, Non human primates
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG1

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted
      See all CD34 primary antibodies

    • Description

      PerCP/Cy5.5® Mouse monoclonal [581] to CD34, prediluted

    • Host species

      Mouse

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Human blood cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab157325 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use 4µl for 106 cells.

    Or 100µl of whole blood.
    ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    Notes
    Flow Cyt
    Use 4µl for 106 cells.

    Or 100µl of whole blood.
    ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

    • Tissue specificity

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

    • Sequence similarities

      Belongs to the CD34 family.

    • Developmental stage

      On early hematopoietic progenitor cells.

    • Post-translational
      modifications

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)
        Flow Cytometry analysis of Human peripheral blood cells labeling CD34 with Anti-CD34 antibody [581], prediluted (PerCP/Cy5.5®) (ab157325).

      References (0)

      Publishing research using ab157325? Please let us know so that we can cite the reference in this datasheet.

      ab157325 has not yet been referenced specifically in any publications.

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