PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [581] to CD34, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human, Non human primates
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG1

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted
      See all CD34 primary antibodies

    • Description

      PerCP/Cy5.5® Mouse monoclonal [581] to CD34, prediluted

    • Host species

      Mouse

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human, Non human primates

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Human blood cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Applications

    Our Abpromise guarantee covers the use of ab157325 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells.

    Or 100µl of whole blood.
    ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

    • Tissue specificity

      Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

    • Sequence similarities

      Belongs to the CD34 family.

    • Developmental stage

      On early hematopoietic progenitor cells.

    • Post-translational
      modifications

      Highly glycosylated.
      Phosphorylated on serine residues by PKC.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD34 antibody
        • CD34 antigen antibody
        • CD34 molecule antibody
        • CD34_HUMAN antibody
        • Cluster designation 34 antibody
        • cluster of differentiation 34 antibody
        • Hematopoietic progenitor cell antigen CD34 antibody
        • HPCA1 antibody
        • Mucosialin antibody
        • OTTHUMP00000034733 antibody
        • OTTHUMP00000034734 antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD34 antibody [581], prediluted (ab157325)
        Flow Cytometry analysis of Human peripheral blood cells labeling CD34 with Anti-CD34 antibody [581], prediluted (PerCP/Cy5.5®) (ab157325).

      References (0)

      Publishing research using ab157325? Please let us know so that we can cite the reference in this datasheet.

      ab157325 has not yet been referenced specifically in any publications.

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