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Immunology Adaptive Immunity B Cells CD
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PerCP/Cy5.5® Anti-CD38 antibody [HIT2] (ab218650)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD38 antibody [HIT2] (ab218650)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [HIT2] to CD38
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG1

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    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD38 antibody [HIT2]
      See all CD38 primary antibodies
    • Description

      PerCP/Cy5.5® Mouse monoclonal [HIT2] to CD38
    • Host species

      Mouse
    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Human peripheral blood lymphocytes.
    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      ab218650 was purified from tissue culture supernatant via affinity chromatography.
    • Clonality

      Monoclonal
    • Clone number

      HIT2
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • NK Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia

    Associated products

    • Alternative Versions

      • APC Anti-CD38 antibody [HIT2], prediluted (ab134399)
      • APC/Cy7® Anti-CD38 antibody [HIT2] (ab233461)
      • PE/Cy7® Anti-CD38 antibody [HIT2] (ab233569)
      • Biotin Anti-CD38 antibody [HIT2] (ab239234)
      • PE Anti-CD38 antibody [HIT2] (ab36422)
      • PerCP Anti-CD38 antibody [HIT2] (ab74688)
    • Isotype control

      • PerCP/Cy5.5® Mouse IgG1, Kappa Monoclonal [MOPC-21] - Isotype Control (ab157226)
    • Recombinant Protein

      • Recombinant Human CD38 protein (ab174013)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab218650 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    Stain cell sample in a final volume of approximately 100 µL.

    Notes
    Flow Cyt
    Use 5µl for 105-8 cells.

    Stain cell sample in a final volume of approximately 100 µL.

    Target

    • Function

      Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
    • Tissue specificity

      Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.
    • Sequence similarities

      Belongs to the ADP-ribosyl cyclase family.
    • Developmental stage

      Preferentially expressed at both early and late stages of the B and T-cell maturation. It is also detected on erythroid and myeloid progenitors in bone marrow, where the level of surface expression was shown to decrease during differentiation of blast-forming unit E to colony-forming unit E.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P28907 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 952 Human
      • Omim: 107270 Human
      • SwissProt: P28907 Human
      • Unigene: 479214 Human
      • Form

        There are 2 isoforms produced by alternative splicing.
      • Alternative names

        • Acute lymphoblastic leukemia cells antigen CD38 antibody
        • ADP ribosyl cyclase 1 antibody
        • ADP ribosyl cyclase antibody
        • ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody
        • ADP-ribosyl cyclase 1 antibody
        • ADPRC 1 antibody
        • ADPRC1 antibody
        • cADPr hydrolase 1 antibody
        • CD 38 antibody
        • CD38 antibody
        • CD38 antigen (p45) antibody
        • CD38 antigen antibody
        • CD38 molecule antibody
        • Cd38-rs1 antibody
        • CD38_HUMAN antibody
        • CD38H antibody
        • Cyclic ADP ribose hydrolase antibody
        • Cyclic ADP ribose hydrolase 1 antibody
        • Cyclic ADP-ribose hydrolase 1 antibody
        • EC 3.2.2.5 antibody
        • Ecto nicotinamide adenine dinucleotide glycohydrolase antibody
        • I-19 antibody
        • I19 (mouse) antibody
        • Lymphocyte differentiation antigen CD38 antibody
        • NAD(+) nucleosidase antibody
        • NIM-R5 antigen antibody
        • NIMR5 antigen (mouse) antibody
        • OTTHUMP00000158633 antibody
        • OTTHUMP00000217743 antibody
        • p45 antibody
        • T10 antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD38 antibody [HIT2] (ab218650)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD38 antibody [HIT2] (ab218650)

        Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD38 with 5 µL (0.25 µg) ab218650 (solid line) or 0.25 µg PerCP-Cyanine5.5 Mouse IgG1 isotype control (dashed line).

         

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab218650? Please let us know so that we can cite the reference in this datasheet.

      ab218650 has not yet been referenced specifically in any publications.

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