PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (ab210265)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (ab210265)](https://www.abcam.com/ps/products/210/ab210265/Images/ab210265-256696-anti-cd4-antibody-gk15-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (ab210265)")
Key features and details
- PerCP/Cy5.5® Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
PerCP/Cy5.5® Anti-CD4 antibody [GK1.5]
See all CD4 primary antibodies -
Description
PerCP/Cy5.5® Rat monoclonal [GK1.5] to CD4 -
Host species
Rat -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Syrian hamster
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Immunogen
Tissue, cells or virus corresponding to Mouse CD4. Mouse CTL clone V4.
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Positive control
- C57Bl/6 splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab210265 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
GK1.5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 0.06 µg. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 0.06 µg. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (ab210265)
Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.06 µg ab210265 (solid line), compared with 0.06 µg Per-Cy5.5 Rat IgG2b isotype control (dashed line).
Datasheets and documents
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Datasheet download
References (0)
ab210265 has not yet been referenced specifically in any publications.
