PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (ab210324)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (ab210324)](https://www.abcam.com/ps/products/210/ab210324/Images/ab210324-257862-anti-cd4-antibody-okt4-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (ab210324)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [OKT4] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Overview
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Product name
PerCP/Cy5.5® Anti-CD4 antibody [OKT4]
See all CD4 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [OKT4] to CD4 -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey
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Immunogen
Tissue, cells or virus corresponding to Human CD4. Sheep Erythrocyte Rosette-purified Human T Cells.
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Positive control
- Human peripheral blood lymphocytes.
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General notes
This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab210324 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
OKT4 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab210324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 105-8 cells. The antibody has been diluted for use at 5 µl (0.25 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µl. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 450124 Chimpanzee
- Entrez Gene: 101864991 Cynomolgus monkey
- Entrez Gene: 920 Human
- Entrez Gene: 713807 Rhesus monkey
- Omim: 186940 Human
- SwissProt: P16004 Chimpanzee
- SwissProt: P79185 Cynomolgus monkey
- SwissProt: P01730 Human
see all -
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (ab210324)
Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD4 with 5 μl (0.25 μg) ab210324 (solid line), compared with 0.25 μg of PerCP/Cy5.5® Mouse IgG2b isotype control (dashed line).
Datasheets and documents
References (1)
ab210324 has been referenced in 1 publication.
- Hu Y et al. Effect of TGF-ß1 on blood CD4+CD25high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis. Exp Ther Med 16:1403-1410 (2018). PubMed: 30112067
