PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)](https://www.abcam.com/ps/products/134/ab134355/Images/ab134355-276710-anti-cd4-antibody-rpa-t4-percp-cy55-flow-cytometry.png "Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [RPA-T4] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
Overview
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Product name
PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4]
See all CD4 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [RPA-T4] to CD4 -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human Peripheral Blood Mononuclear Cells (PBMCs) stimulated with PHA.
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Positive control
- Human peripheral blood cells
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General notes
The RPA-T4 clone blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
The RPA-T4 clone blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. -
Clonality
Monoclonal -
Clone number
RPA-T4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)
Flow cytometry of human peripheral blood lymphocytes labeling CD4 with ab134355 at 0.125 ug (solid line), or Mouse IgG1 isotype control at 0.125 ug (dashed line).
Datasheets and documents
References (0)
ab134355 has not yet been referenced specifically in any publications.
