PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)
![Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)](https://www.abcam.com/ps/products/134/ab134355/Images/ab134355-276710-anti-cd4-antibody-rpa-t4-percp-cy55-flow-cytometry.png "Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [RPA-T4] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
Overview
-
Product name
PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4]
See all CD4 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [RPA-T4] to CD4 -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human Peripheral Blood Mononuclear Cells (PBMCs) stimulated with PHA.
-
Positive control
- Human peripheral blood cells
-
General notes
The RPA-T4 clone blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
The RPA-T4 clone blocks HIV binding and mixed lymphocyte reaction. RPA-T4 recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. -
Clonality
Monoclonal -
Clone number
RPA-T4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
-
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
-
Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [RPA-T4] (ab134355)
Flow cytometry of human peripheral blood lymphocytes labeling CD4 with ab134355 at 0.125 ug (solid line), or Mouse IgG1 isotype control at 0.125 ug (dashed line).
Datasheets and documents
-
Datasheet download
References (0)
ab134355 has not yet been referenced specifically in any publications.
