Key features and details
- PerCP/Cy5.5® Rat monoclonal [IM7] to CD44
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Product namePerCP/Cy5.5® Anti-CD44 antibody [IM7]
See all CD44 primary antibodies
DescriptionPerCP/Cy5.5® Rat monoclonal [IM7] to CD44
ConjugationPerCP/Cy5.5®. Ex: 482nm, Em: 690nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Horse, Cat, Dog, Human
Tissue, cells or virus. (Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1).
- Flow Cyt: Mouse splenocytes.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.0975% Sodium azide
Concentration information loading...
Purification notesPurified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Our Abpromise guarantee covers the use of ab234445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 µg/ml.|
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
- Information by UniProt
- LHR antibody
- BA-1 antibody
- CD 44 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234445 has not yet been referenced specifically in any publications.