PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted (ab155362)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted (ab155362)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [HI100] to CD45RA, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG2b

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted
      See all CD45RA primary antibodies

    • Description

      PerCP/Cy5.5® Mouse monoclonal [HI100] to CD45RA, prediluted

    • Host species

      Mouse

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Normal Human peripheral blood cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab155362 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (1)
    Use 5µl for 106 cells.
    Notes
    Flow Cyt
    Use 5µl for 106 cells.

    Target

    • Function

      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

    • Involvement in disease

      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis

    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.

    • Domain

      The first PTPase domain interacts with SKAP1.

    • Post-translational
      modifications

      Heavily N- and O-glycosylated.

    • Cellular localization

      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt

    • Database links

      • Alternative names

        • B220 antibody
        • CD45 antibody
        • CD45 antigen antibody
        • CD45R antibody
        • EC 3.1.3.48 antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • Loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lymphocyte antigen 5 antibody
        • Lyt-4 antibody
        • Protein tyrosine phosphatase, receptor type C antibody
        • Protein tyrosine phosphatase, receptor type, C antibody
        • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • RT7 antibody
        • T200 antibody
        • T200 glycoprotein antibody
        • T200 leukocyte common antigen antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted (ab155362)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD45RA antibody [HI100], prediluted (ab155362)
        Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG2b kappa Isotype Control PerCP-Cy5.5 (open histogram) or f ab155362 PerCP-Cy5.5 (filled histogram). Cells in the lymphocyte gate were used for analysis.

      References (0)

      Publishing research using ab155362? Please let us know so that we can cite the reference in this datasheet.

      ab155362 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Flow Cytometry abreview for Anti-CD45RA antibody [HI100], prediluted (PerCP/Cy5.5®)

       Good
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Permeabilization
      No
      Sample
      Human Cell (PBMC)
      Specification
      PBMC
      Gating Strategy
      lymphocytes
      Preparation
      Cell harvesting/tissue preparation method: Ficoll
      Sample buffer: DPBS
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 17 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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