PerCP/Cy5.5® Anti-CD8 antibody [MEM-31] (ab157306)

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Flow Cytometry - PerCP/Cy5.5® Anti-CD8 antibody [MEM-31] (ab157306)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [MEM-31] to CD8
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG2a

    Overview

    • Product name

      PerCP/Cy5.5® Anti-CD8 antibody [MEM-31]
      See all CD8 primary antibodies

    • Description

      PerCP/Cy5.5® Mouse monoclonal [MEM-31] to CD8

    • Host species

      Mouse

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: WB

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Crude thymus membrane fraction.

    • Epitope

      ab157306 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.

    • General notes

      ab157306 does not react with formaldehyde-fixed cells.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab157306 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells. (or 100 µl of whole blood).
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      • Form

        CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.

      • Alternative names

        • CD8 antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8a antigen antibody
        • CD8a molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD8 antibody [MEM-31] (ab157306)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD8 antibody [MEM-31] (ab157306)
        Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP-CyTM5.5.

      References (0)

      Publishing research using ab157306? Please let us know so that we can cite the reference in this datasheet.

      ab157306 has not yet been referenced specifically in any publications.

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