PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)

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Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)

    Key features and details

    • PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG2b

    Overview

    • Product name

      PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2]
      See all H2-Ea primary antibodies

    • Description

      PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea

    • Host species

      Rat

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Specificity

      ab218783 reacts with Mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. It does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • C57Bl/6 splenocytes.

    • General notes

       This product was previously labelled as MHC Class II IA + IE

       

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.87% Sodium chloride, 0.1% Gelatin, 0.12% Monobasic dihydrogen sodium phosphate
    • Concentration information loading...

    • Purity

      Protein A purified

    • Purification notes

      ab218783 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    • Clonality

      Monoclonal

    • Clone number

      M5/114.15.2

    • Isotype

      IgG2b

    • Light chain type

      kappa

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab218783 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Use 0.25 µg.

    Target

    • Relevance

      The MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

    • Cellular localization

      Single-pass type I membrane protein

    • Database links

      • Alternative names

        • AI323765 antibody
        • E alpha f antibody
        • H 2Ea antibody
        • H 2I antibody
        • H2 Ea antibody
        • H2 I antibody
        • Histocompatibility 2, class II antigen E alpha antibody
        • I E alpha MHC class II antibody
        • Ia 3 antibody
        • Ia3 antibody
        • MGC117888 antibody
        • MHC class II antigen E alpha antibody
        • MHC class II protein antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)
        Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)

        Flow cytometric analysis of C57Bl/6 splenocytes labeling H2-Ea with 0.25µg ab218783 (solid line), compared with 0.25µg PerCP/Cy5.5® Rat IgG2b isotype control (dashed line).

      References (0)

      Publishing research using ab218783? Please let us know so that we can cite the reference in this datasheet.

      ab218783 has not yet been referenced specifically in any publications.

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