PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)
![Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)](https://www.abcam.com/ps/products/218/ab218783/Images/ab218783-274502-anti-mhc-class-ii-ia-ie-antibody-m5-114152-percp-cy55-flow-cytometry.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)")
Key features and details
- PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Overview
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Product name
PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2]
See all H2-Ea primary antibodies -
Description
PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea -
Host species
Rat -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Specificity
ab218783 reacts with Mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. It does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- C57Bl/6 splenocytes.
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General notes
This product was previously labelled as MHC Class II IA + IE
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.1% Gelatin, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab218783 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
M5/114.15.2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 0.25 µg. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 0.25 µg. |
Target
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Relevance
The MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. -
Cellular localization
Single-pass type I membrane protein -
Database links
- Entrez Gene: 111861 Mouse
- SwissProt: P01904 Mouse
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Alternative names
- AI323765 antibody
- E alpha f antibody
- H 2Ea antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)
Flow cytometric analysis of C57Bl/6 splenocytes labeling H2-Ea with 0.25µg ab218783 (solid line), compared with 0.25µg PerCP/Cy5.5® Rat IgG2b isotype control (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab218783 has not yet been referenced specifically in any publications.