PerCP/Cy5.5® Anti-HLA-DR antibody [L243] (ab157330)
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [L243] to HLA-DR
- Suitable for: Flow Cyt
- Reacts with: Dog, Human, Non human primates
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PerCP/Cy5.5® Anti-HLA-DR antibody [L243]
See all HLA-DR primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [L243] to HLA-DR -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Specificity
ab157330 recognizes specifically HLA-DR molecules, both peptide-loaded and empty. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Dog, Human, Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human HLA-DR. Human B lymphocytes
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Positive control
- Human blood cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 99.7% PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye PerCP-Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
L243 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
- Anti-HLA-DR antibody [L243] (ab136320)
- FITC Anti-HLA-DR antibody [L243] (ab210298)
- Alexa Fluor® 647 Anti-HLA-DR antibody [L243] (ab239277)
- PE/Cy5® Anti-HLA-DR antibody [L243] (ab239296)
- APC/Cy7® Anti-HLA-DR antibody [L243] (ab239308)
- PE/Cy7® Anti-HLA-DR antibody [L243] (ab239318)
- PE/DyLight™ 594 Anti-HLA-DR antibody [L243] (ab239320)
- redFluor™ 710 Anti-HLA-DR antibody [L243] (ab253079)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 4µl for 106 cells.
Use 4ul per 100ul whole blood. |
Notes |
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Flow Cyt
Use 4µl for 106 cells. Use 4ul per 100ul whole blood. |
Target
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Function
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. -
Sequence similarities
Belongs to the MHC class II family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsUbiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. - Information by UniProt
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Database links
- Entrez Gene: 3122 Human
- Omim: 142860 Human
- SwissProt: P01903 Human
- Unigene: 520048 Human
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Alternative names
- DASS-397D15.1 antibody
- DR alpha chain antibody
- DR alpha chain precursor antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157330 has not yet been referenced specifically in any publications.