Key features and details
- PerCP/Cy5.5® Mouse monoclonal [NKTA255] to LAIR1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
Product namePerCP/Cy5.5® Anti-LAIR1 antibody [NKTA255]
See all LAIR1 primary antibodies
DescriptionPerCP/Cy5.5® Mouse monoclonal [NKTA255] to LAIR1
ConjugationPerCP/Cy5.5®. Ex: 482nm, Em: 690nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human LAIR1. Activated NK cells and CD3- thymocytes.
- Flow Cytometry: Human peripheral blood.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.0975% Sodium azide
Concentration information loading...
Purification notesab234263 is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Our Abpromise guarantee covers the use of ab234263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µl for 106 cells.
(or 100 μL whole blood)
FunctionFunctions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
Tissue specificityExpressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Developmental stageComplete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
DomainITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
modificationsPhosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
Cellular localizationCell membrane.
- Information by UniProt
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234263 has not yet been referenced specifically in any publications.