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PerCP/Cy5.5® Anti-Ly6g antibody [1A8] (ab210207)

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Flow Cytometry - PerCP/Cy5.5® Anti-Ly6g antibody [1A8] (ab210207)

    Key features and details

    • PerCP/Cy5.5® Rat monoclonal [1A8] to Ly6g
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)
    Protein
    Product image
    Recombinant Mouse Ly6g protein (His tag) (ab241265)

    View more associated products

    Overview

    • Product name

      PerCP/Cy5.5® Anti-Ly6g antibody [1A8]
      See all Ly6g primary antibodies
    • Description

      PerCP/Cy5.5® Rat monoclonal [1A8] to Ly6g
    • Host species

      Rat
    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • C57Bl/6 bone marrow cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.87% Sodium chloride, 0.1% Gelatin, 0.12% Monobasic dihydrogen sodium phosphate
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab210207 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
    • Clonality

      Monoclonal
    • Clone number

      1A8
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage

    Associated products

    • Alternative Versions

      • APC Anti-Ly6g antibody [1A8] (ab210201)
      • FITC Anti-Ly6g antibody [1A8] (ab210203)
      • Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)
      • PE/Cy7® Anti-Ly6g antibody [1A8] (ab210206)
      • CF405M Anti-Ly6g antibody [1A8] (ab236484)
      • PerCP Anti-Ly6g antibody [1A8] (ab236485)
      • redFluor™ 710 Anti-Ly6g antibody [1A8] (ab242066)
      • violetFluor™ 450 Anti-Ly6g antibody [1A8] (ab242074)
    • Recombinant Protein

      • Recombinant Mouse Ly6g protein (His tag) (ab241265)

    Applications

    Our Abpromise guarantee covers the use of ab210207 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Suggested use 0.25 μg.

    Target

    • Relevance

      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
    • Cellular localization

      Attached to the membrane by a GPI-anchor.
    • Database links

      • Entrez Gene: 546644 Mouse
      • SwissProt: P35461 Mouse
      • Alternative names

        • Gr-1 antibody
        • Gr1 antibody
        • Ly-6G.1 antibody
        • Ly6G antibody
        • Lymphocyte antigen 6 complex locus G antibody
        • Lymphocyte antigen 6G antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-Ly6g antibody [1A8] (ab210207)
        Flow Cytometry - PerCP/Cy5.5® Anti-Ly6g antibody [1A8] (ab210207)

        Flow cytometric analysis of C57Bl/6 mouse bone marrow cells labeling Ly6g with ab210207 at 0.25 µg (solid line) or PerCP/Cy5.5® rat IgG2a  isotype control at 0.25 µg (dashed line).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab210207? Please let us know so that we can cite the reference in this datasheet.

      ab210207 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab210207.
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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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