PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (ab218775)
![Flow Cytometry - PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (ab218775)](https://www.abcam.com/ps/products/218/ab218775/Images/ab218775-274531-218775.JPG "Flow Cytometry - PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (ab218775)")
Key features and details
- PerCP/Cy5.5® Rat monoclonal [30-H12] to Thy1.2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Overview
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Product name
PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12]
See all Thy1.2 primary antibodies -
Description
PerCP/Cy5.5® Rat monoclonal [30-H12] to Thy1.2 -
Host species
Rat -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57Bl/6 splenocytes.
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General notes
Thy1.2 is expressed on thymocytes, mature T cells and neurons in mouse strains that express the CD90.2 allele (BALB/c, CBA, C3H, C57BL/6, SJL and others). ab218775 does not react with the CD90.1 allele expressed in mouse strains such as PL and AKR.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab218775 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
30-H12 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 0.25µg per reaction. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 0.25µg per reaction. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
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Alternative names
- CD90 antibody
- CD90 antigen antibody
- CD90.2 antibody
see all
Images
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Flow Cytometry - PerCP/Cy5.5® Anti-Thy1.2 antibody [30-H12] (ab218775)
Flow Cytometry analysis of C57Bl/6 splenocytes labeling Thy1.2 with ab218775 at 0.25 µg (solid line) or 0.25 µg PerCP-Cy5.5® Rat IgG2b isotype control (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab218775 has not yet been referenced specifically in any publications.