PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75] (ab239340)

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Flow Cytometry - PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75] (ab239340)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [MEM-75] to Transferrin Receptor
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG1

    Overview

    • Product name

      PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75]
      See all Transferrin Receptor primary antibodies

    • Description

      PerCP/Cy5.5® Mouse monoclonal [MEM-75] to Transferrin Receptor

    • Host species

      Mouse

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human Transferrin Receptor. (NALM-6 human pre-B cell line).

    • Positive control

      • Flow Cyt: K562 cells.

    • General notes

      The antibody MEM-75 does not block binding of transferrin to the receptor.

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab239340 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    (or 100 μl of whole blood).

    Target

    • Function

      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

    • Involvement in disease

      Immunodeficiency 46

    • Sequence similarities

      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.

    • Post-translational
      modifications

      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

    • Cellular localization

      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 71 antibody
        • CD71 antibody
        • CD71 antigen antibody
        • IMD46 antibody
        • OTTHUMP00000208523 antibody
        • OTTHUMP00000208524 antibody
        • OTTHUMP00000208525 antibody
        • p90 antibody
        • sTfR antibody
        • T9 antibody
        • TFR 1 antibody
        • TfR antibody
        • TfR1 antibody
        • TFR1_HUMAN antibody
        • TFRC antibody
        • TR antibody
        • Transferrin receptor (p90 CD71) antibody
        • Transferrin receptor protein 1, serum form antibody
        • Trfr antibody
        see all

      Images

      • Flow Cytometry - PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75] (ab239340)
        Flow Cytometry - PerCP/Cy5.5® Anti-Transferrin Receptor antibody [MEM-75] (ab239340)

        Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Transferrin Receptor with ab239340. Surface staining.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab239340? Please let us know so that we can cite the reference in this datasheet.

      ab239340 has not yet been referenced specifically in any publications.

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