Product namePerCP/Cy5.5® Conjugation Kit - Lightning-Link®
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 763-0010 was previously called Lightning-Link® PerCP/Cy5.5 Labeling Kit 3 reactions each up to 100 µg and is the same as the 3 x 100 µg size of this product. Expedeon product code 763-0030 was previously called Lightning-Link® PerCP/Cy5.5 Labeling Kit 3 reactions each up to 10 µg and is the same as the 3 x 10 µg size of this product. Expedeon product code 763-0005 was previously called Lightning-Link® PerCP/Cy5.5 Labeling Kit 1 reaction up to 100 µg and is the same as the 100 µg size of this product. Expedeon product code 763-0015 was previously called Lightning-Link® PerCP/Cy5.5 Labeling Kit 1 reaction up to 1 mg and is the same as the 1 mg size of this product.
PerCP/Cy5.5® Conjugation Kit / PerCP/Cy5.5® Labeling Kit ab102911 uses a simple and quick process for PerCP/Cy5.5 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to PerCP/Cy5.5® using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The PerCP/Cy5.5® conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to PerCP/Cy5.5®.
Amount and volume of antibody for conjugation to PerCP/Cy5.5®
Kit size Recommended maximum
amount of antibody
30 µg 3 x 10 µg 3 x 10 µL 100 µg 100 µg 100 µL 300 µg 3 x 100 µg 3 x 100 µL 1 mg 1 mg 1 mL
1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture.
Tested applicationsSuitable for: Conjugationmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg 300 µg 30 µg Modifier reagent 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial PerCP/Cy5.5 mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg 3 x 100µg 3 x 10µg Quencher reagent 1 vial 1 vial 1 vial 1 vial 1 vial 1 vial
- Peridinin chlorophyll protein complex
Our Abpromise guarantee covers the use of ab102911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent dilution.|
This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.
Maekawa N et al. used ab102911 as part of examining a canine chimeric monoclonal antibody targeting PD-L1.
They used the kit to conjugate PerCP/Cy5.5® to anti-canine CD4 antibody for use in flow cytometry.
To evaluate cell proliferation, nucleotide analogue 5-ethynyl-2′-deoxyuridine (EdU) was added to the medium on day 2, and cells were harvested after incubation for another 2 h. The lymphocyte population was gated by forward scatter and side scatter, and the incorporation of EdU in (c) CD4+ cells was measured by a flow cytometer. Statistical analysis was performed with a Wilcoxon signed rank-sum test.