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Hi, 1) The concentration was 1mg/ml and 100ug was used for the conjugation reaction. 2) The antibody was in PBS at ph 7.4 with no other additives. That would be the purpose of the conjugation kit. This antibody would be nice to have conjugated to PerCP-Cy5.5 based on the antibodies that we can buy commercially already have different tags. Thanks,
Asked on Jan 17 2012
Thank you for getting back to me and for providing some further information. Your co-operation is much appreciated. I understand that there is no additive in the storage buffer which could interact with the conjugation reaction. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. I could offer you either a new kit as free of charge or a credit note which you could use in the future. Please do let me know how you wish to proceed. I look forward to hearing from you soon.
Answered on Jan 17 2012