PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)

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Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)

    Overview

    • Product name

      PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control

    • Description

      PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control

    • Conjugation

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • Specificity

      This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

    • Immunogen

      Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. (KLH-coupled trinitrophenol).

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purified antibody is conjugated with PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    • Clonality

      Monoclonal

    • Clone number

      MPC-11

    • Isotype

      IgG2b

    • Light chain type

      kappa

    Associated products

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab239460 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt
      Use at an assay dependent concentration.

      The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
      Notes
      Flow Cyt
      Use at an assay dependent concentration.

      The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

      Images

      • Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)
        Flow Cytometry - PerCP/Cy5.5® Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab239460)

        Example of nonspecific ab239460 signal on human peripheral blood; surface staining, 1 μg/ml.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab239460? Please let us know so that we can cite the reference in this datasheet.

      ab239460 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Q&A
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