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    perforin-antibody-5b10-ab89821.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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Anti-Perforin antibody [5B10] (ab89821)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (ab89821)

    Key features and details

    • Mouse monoclonal [5B10] to Perforin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Perforin antibody [5B10]
      See all Perforin primary antibodies
    • Description

      Mouse monoclonal [5B10] to Perforin
    • Host species

      Mouse
    • Specificity

      This antibody is specific to a 70 kDa protein, which is a potent cytolytic pore-forming protein.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment corresponding to an external region of the C terminal of Human perforin.

    • Positive control

      • Spleen
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      5B10
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • Cytotoxic Cells
      • Immunology
      • Innate Immunity
      • Complement
      • Other

    Associated products

    • Alternative Versions

      • Anti-Perforin antibody [5B10], prediluted (ab75573)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Perforin protein (ab114201)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab89821 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/10 - 1/20.
    Notes
    IHC-P
    1/10 - 1/20.

    Target

    • Function

      Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
    • Involvement in disease

      Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • Sequence similarities

      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 C2 domain.
      Contains 1 EGF-like domain.
      Contains 1 MACPF domain.
    • Domain

      The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
    • Target information above from: UniProt accession P14222 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5551 Human
      • Omim: 170280 Human
      • SwissProt: P14222 Human
      • Unigene: 2200 Human
      • Alternative names

        • Cytolysin antibody
        • FLH2 antibody
        • HPLH2 antibody
        • Lymphocyte pore-forming protein antibody
        • P1 antibody
        • PERF_HUMAN antibody
        • perforin 1 (pore forming protein) antibody
        • Perforin 1 antibody
        • Perforin-1 antibody
        • PFP antibody
        • PGFL antibody
        • PIGF antibody
        • PIGF-2 antibody
        • PLGF antibody
        • Pore forming protein antibody
        • prf1 antibody
        • SHGC-10760 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (ab89821)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (ab89821)

        Immunohistochemical analysis of human spleen labeling Perforin with ab89821 at 1:20 dilution. Secondary antibody was RTU PolyVue Plus HRP.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab89821? Please let us know so that we can cite the reference in this datasheet.

      ab89821 has been referenced in 2 publications.

      • Yao S  et al. CD4+ T cells contain early extrapulmonary tuberculosis (TB) dissemination and rapid TB progression and sustain multieffector functions of CD8+ T and CD3- lymphocytes: mechanisms of CD4+ T cell immunity. J Immunol 192:2120-32 (2014). PubMed: 24489088
      • Alkins R  et al. Focused ultrasound delivers targeted immune cells to metastatic brain tumors. Cancer Res 73:1892-9 (2013). IHC-P ; Human . PubMed: 23302230

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