Overview

  • Product name
    Anti-Perforin antibody [5B10], prediluted
    See all Perforin primary antibodies
  • Description
    Mouse monoclonal [5B10] to Perforin, prediluted
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the C terminal region of human Perforin.

  • Positive control
    • Lymphoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab75573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • Involvement in disease
    Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • Sequence similarities
    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • Domain
    The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lymphoma stained with neat ab75573, using peroxidase-conjugate and AEC chromogen. Note granular cytoplasmic staining of lymphocytes.
  • Immunohistochemical analysis of Human skin tissue, staining Perforin with ab75573.

    Tissue was fixed with paraformaldehyde and permeabilized with Tween-20; antigen retrieval was by heat mediation in Tris-EDTA buffer. Samples were incubated with primary antibody (undiluted) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Koelzer VH  et al. Digital analysis and epigenetic regulation of the signature of rejection in colorectal cancer. Oncoimmunology 6:e1288330 (2017). Read more (PubMed: 28507795) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for your patience whilst I contacted the lab for more information regarding ab75573.

The Perforin datasheet indicates that the antibody was raised to a recombinant prokaryotic protein corresponding to an external region of the C-terminus of the perforin molecule.

The Design History File provides more specific information to indicate that a recombinant protein of approximately 23 kd was expressed that avoided the EGF-like domain, the C2 type domain and the membrane-spanning domains. The antibody has not been epitope mapped and so we do not know the exact amino acid sequence to which the antibody binds. However, as isoforms of the perforin protein have been reported to contain substitutions in the C2 domain it is likely that our antibody will detect all known isoforms of the perforin protein.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam.

I have inquired about the Anti-Perforin antibody [5B10] but as it is manufactured off site, I will need to do some more searching to find the necessary information and also to determine if we do know which amino acids the antibody binds to.

I will get back in contact as soon as I have found out any more information.

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Question
Answer

Thank you for contacting us.


As discussed over the phone, anti-Perforin antibody (ab75573) is a preparation of tissue culture supernatant, and as such the exact concentration cannot be accurately determined. It has however been estimated to be within a range of 0.5-1 ug/mL.


I hope this has been of help. If you require any further information please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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