Key features and details
- Mouse monoclonal [B-D48] to Perforin
- Suitable for: WB, Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Perforin antibody [B-D48]
See all Perforin primary antibodies
DescriptionMouse monoclonal [B-D48] to Perforin
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant human perforin.
- IHC-P: Human spleen FFPE tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Sterile-filtered through 0.22 µm and treated to remove endotoxins.
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab47225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use a concentration of 20 µg/ml.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Involvement in diseaseDefects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain.
DomainThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
Cellular localizationCytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
- Information by UniProt
- Cytolysin antibody
- FLH2 antibody
- HPLH2 antibody
IHC image of Perforin staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47225, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab47225 has been referenced in 10 publications.
- Konjevic Sabolek M et al. Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis. Ann Clin Transl Neurol 6:1151-1164 (2019). PubMed: 31353869
- Egui A et al. A Parasite Biomarker Set for Evaluating Benznidazole Treatment Efficacy in Patients with Chronic Asymptomatic Trypanosoma cruzi Infection. Antimicrob Agents Chemother 63:N/A (2019). PubMed: 31358581
- Bengsch B et al. Deep immune profiling by mass cytometry links human T and NK cell differentiation and cytotoxic molecule expression patterns. J Immunol Methods 453:3-10 (2018). PubMed: 28322863
- Wakiyama H et al. Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC). Anticancer Res 38:6631-6638 (2018). PubMed: 30504371
- Flerin NC et al. T-Cell Receptor (TCR) Clonotype-Specific Differences in Inhibitory Activity of HIV-1 Cytotoxic T-Cell Clones Is Not Mediated by TCR Alone. J Virol 91:N/A (2017). Mass Cytometry ; Human . PubMed: 28077649
- Nagy E et al. Interferon-? Released by Activated CD8+ T Lymphocytes Impairs the Calcium Resorption Potential of Osteoclasts in Calcified Human Aortic Valves. Am J Pathol 187:1413-1425 (2017). IHC-Fr ; Human . PubMed: 28431214
- Yuan YH et al. NaHCO3 enhances the antitumor activities of cytokine-induced killer cells against hepatocellular carcinoma HepG2 cells. Oncol Lett 12:3167-3174 (2016). IHC-P . PubMed: 27899977
- Liu S et al. Recruitment of Grb2 and SHIP1 by the ITT-like motif of TIGIT suppresses granule polarization and cytotoxicity of NK cells. Cell Death Differ : (2012). PubMed: 23154388
- Trotta R et al. The PP2A inhibitor SET regulates granzyme B expression in human natural killer cells. Blood 117:2378-84 (2011). WB ; Human . PubMed: 21156847
- Schmidt H et al. Enrichment and analysis of secretory lysosomes from lymphocyte populations. BMC Immunol 10:41 (2009). WB ; Human . PubMed: 19640298