• Product name
    Anti-Perforin antibody [CE2.10]
    See all Perforin primary antibodies
  • Description
    Mouse monoclonal [CE2.10] to Perforin
  • Host species
  • Specificity
    The antibody does not detect human perforin by Western blot.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 98 - 534 of Mouse Perforin.

  • Epitope
    Recognizes an epitope in the region amino acids 402-534 of human and mouse perforin.
  • Positive control
    • Mouse T cell clone B6.1


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    This antibody is produced in the medium called optimem with no serum, therefore it does not have to be purified.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab16079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at an assay dependent dilution on human and mouse samples.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa) from the mouse T cell clone B6.1.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
    • Involvement in disease
      Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • Sequence similarities
      Belongs to the complement C6/C7/C8/C9 family.
      Contains 1 C2 domain.
      Contains 1 EGF-like domain.
      Contains 1 MACPF domain.
    • Domain
      The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
    • Post-translational
    • Cellular localization
      Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytolysin antibody
      • FLH2 antibody
      • HPLH2 antibody
      • Lymphocyte pore-forming protein antibody
      • P1 antibody
      • PERF_HUMAN antibody
      • perforin 1 (pore forming protein) antibody
      • Perforin 1 antibody
      • Perforin-1 antibody
      • PFP antibody
      • PGFL antibody
      • PIGF antibody
      • PIGF-2 antibody
      • PLGF antibody
      • Pore forming protein antibody
      • prf1 antibody
      • SHGC-10760 antibody
      see all


    ab16079 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for your email. You are correct, this antibody does not detect human perforin by Western blot. I do apologize as I did not see that statement at first on the online datasheet, and I have updated the datasheet to make it more obvious. I would therefore not recommend purchasing this antibody to use in Western blotting with human samples. We don't have another perforin antibody that has been tested for application in Western blotting and cross-reacts with human. Ab16074 and ab7203 work in WB with mouse samples, but have not been tested for cross-reactivity with human. Ab1814 reacts with human but has not yet been tested in Western blotting. If you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). If you have any additional questions, please contact us again.

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