Overview

  • Product name

    Anti-Perforin antibody [dG9]
    See all Perforin primary antibodies
  • Description

    Mouse monoclonal [dG9] to Perforin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, IHC-Fr, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Cow, Human
    Does not react with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Human Perforin. (Purified granules from Human YT lymphoma cell line).

  • General notes

    The mouse monoclonal antibody dG9 is also known as deltaG9.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab194807 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    dG9
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

(Intracellular staining).

 

 

 

 

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • Involvement in disease

    Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
  • Sequence similarities

    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • Domain

    The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytolysin antibody
    • FLH2 antibody
    • HPLH2 antibody
    • Lymphocyte pore-forming protein antibody
    • P1 antibody
    • PERF_HUMAN antibody
    • perforin 1 (pore forming protein) antibody
    • Perforin 1 antibody
    • Perforin-1 antibody
    • PFP antibody
    • PGFL antibody
    • PIGF antibody
    • PIGF-2 antibody
    • PLGF antibody
    • Pore forming protein antibody
    • prf1 antibody
    • SHGC-10760 antibody
    see all

References

This product has been referenced in:

See 1 Publication for this product

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