Key features and details
- Rabbit polyclonal to Pericentrin 1/FROUNT
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Pericentrin 1/FROUNT antibody
See all Pericentrin 1/FROUNT primary antibodies
DescriptionRabbit polyclonal to Pericentrin 1/FROUNT
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- 293T, HeLa, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates.
Protein previously labeled as Pericentrin 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176801 was affinity purified using an epitope specific to Pericentrin 1/FROUNT immobilized on solid support.
Our Abpromise guarantee covers the use of ab176801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEssential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.
Sequence similaritiesBelongs to the nucleoporin Nup85 family.
Cellular localizationNucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Nucleus membrane. During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells.
- Information by UniProt
- 85 kDa nucleoporin antibody
- FLJ12549 antibody
- FROUNT antibody
All lanes : Anti-Pericentrin 1/FROUNT antibody (ab176801) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 3 minutes
Detection of Pericentrin 1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176801 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds..
ab176801 has not yet been referenced specifically in any publications.