Key features and details
- Rabbit polyclonal to Pericentrin 1/FROUNT
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Pericentrin 1/FROUNT antibody
See all Pericentrin 1/FROUNT primary antibodies
DescriptionRabbit polyclonal to Pericentrin 1/FROUNT
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human
Recombinant fragment (His-T7-tag) corresponding to Mouse Pericentrin 1/FROUNT aa 418-653. N-terminal tags. Expressed in E.coli.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-QLGVDYFDCPELG RVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVKSICKILAMKAVRNN RLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGSAMML SDRLTFLGKYREFHRLYGEKRFGDAASLLLSLMTSQIAPRSFWMTLLTDA LPLLEQKQVIFSAEQTYELMRCLEDLASGRPECGEPDAQRLQDDDIETTK VEMLRLALARNLARAIIREGSL
Database link: Q8R480
- IHC-P: Mouse testis, stomach, heart and uterus tissues. WB: Recombinant human Pericentrin 1/FROUNT protein; Mouse lung liver and heart tissues.
Protein previously labeled as Pericentrin 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionEssential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.
Sequence similaritiesBelongs to the nucleoporin Nup85 family.
Cellular localizationNucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Nucleus membrane. During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells.
- Information by UniProt
- 85 kDa nucleoporin antibody
- FLJ12549 antibody
- FROUNT antibody
All lanes : Anti-Pericentrin 1/FROUNT antibody (ab231958) at 2 µg/ml
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse heart tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse heart tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Pericentrin 1/FROUNT using ab231958 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Pericentrin 1/FROUNT antibody (ab231958) at 2 µg/ml + Recombinant human Pericentrin 1/FROUNT protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231958 has not yet been referenced specifically in any publications.